电针通过rACC谷氨酸能神经元缓解炎性痛伴发焦虑样行为  被引量:5

Electroacupuncture alleviates the inflammatory pain-induced anxiety-like behaviors by glutamatergic neurons in rostral anterior cingulate cortex

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作  者:肖思琦 吴泽民[1] 沈醉 孙海榉 朱怡晨 吴梦薇 朱希潇 方剑乔[1] 邵晓梅[1] XIAO Siqi;WU Zemin;SHEN Zui;SUN Haiju;ZHU Yichen;WU Mengwei;ZHU Xixiao;FANG Jianqiao;SHAO Xiaomei(Key Laboratory of Acupuncture and Neurology of Zhejiang province,the Third Clinical Medical College,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区:[1]浙江中医药大学附属第三医院第三临床医学院浙江省针灸神经病学研究重点实验室,杭州310053

出  处:《中国疼痛医学杂志》2022年第7期494-501,共8页Chinese Journal of Pain Medicine

基  金:国家自然科学基金(82074518);浙江省自然科学基金(LY21H270010)。

摘  要:目的:探讨吻侧前扣带皮质(rostral anterior cingulate cortex,rACC)谷氨酸能神经元活性是否与慢性炎性痛伴发焦虑样行为相关,明确电针是否通过调控rACC谷氨酸能神经元活性起到抗焦虑样行为作用。方法:足底皮下注射完全弗氏佐剂(complete Freund's adjuvant,CFA)0.1 ml建立大鼠慢性炎性痛模型;通过注射化学遗传病毒调控rACC谷氨酸能神经元活性,并使用动态足底触觉仪和旷场实验(open field test,OFT)分别检测其机械缩足反射阈值(mechanical withdrawal threshold,MWT)和焦虑样行为改变;观察调控rACC谷氨酸能神经元是否对电针(electroacupuncture,EA)足三里、三阴交穴的抗焦虑样行为作用产生影响。结果:特异性激活生理状态大鼠rACC谷氨酸能神经元可以诱发焦虑样行为;特异性抑制rACC谷氨酸能神经元可缓解CFA模型大鼠的焦虑样行为;电针缓解CFA大鼠焦虑样行为的作用被特异性激活rACC谷氨酸能神经元逆转。结论:电针通过下调rACC谷氨酸能神经元活性缓解慢性炎性痛大鼠的焦虑样行为。Objective:To explore the mechanism of glutamatergic neurons in the rostral anterior cingulate cortex(rACC)in regulating hyperalgesia and pain-induced anxiety-like behavior of chronic inflammatory pain model rats,and to investigate the effect of electroacupuncture intervention.Methods:The chronic inflammatory pain model was constructed by injecting 0.1 ml emulsified CFA into the left hind plantar.Neuronal activity was regulated by injection of adeno-associated virus.Mechanical withdrawal threshold(MWT)and anxiety-like behaviors were measured by dynamic plantar tactile instrument and OFT respectively.It was investigated that the activation of rACC glutamatergic neurons affects the electroacupuncture(EA)intervention on pain related anxiety-like behaviors by stimulating the acupoint of"Zusanli"(ST36)and"Sanyinjiao"(SP6).Results:Specific activation of glutaminergic neurons in the rACC induced anxiety-like behaviors in normal rats.The glutamatergic neurons in the rACC inhibited specifically could alleviated the pain-induced anxiety-like behaviors in CFA model rats.The antianxietic effect of EA was reversed by specifically activating glutamatergic neurons in the rACC.Conclusion:Electroacupuncture alleviates the chronic inflammatory pain-induced anxiety-like behaviors by decreasing the activity of glutamatergic neurons in the rostral anterior cingulate cortex.

关 键 词:吻侧前扣带皮质 谷氨酸能神经元 慢性疼痛 焦虑样行为 电针 

分 类 号:R245.97[医药卫生—针灸推拿学]

 

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