小鼠深静脉血栓实验模型的制备  

Preparation of experimental animal model of mouse deep vein thrombosis

作　　者：张茜[1,2,3] 谷田 刘哲宇[1,2,3] 李思 郑晓新 ZHANG Xi;GU Tian;LIU Zheyu;LI Si;ZHENG Xiaoxin(Department of Cardiovascular Medicine,Renmin Hospital of Wuhan University,Wuhan Hubei 430060;Hubei Key Laboratory of Cardiovascular Diseases,Wuhan Hubei 430060;Institute of Cardiovascular Disease,Wuhan University,Wuhan Hubei 430060,China)

机构地区：[1]武汉大学人民医院心血管内科,湖北武汉430060 [2]心血管病湖北省重点实验室,湖北武汉430060 [3]武汉大学心血管病研究所,湖北武汉430060

出　　处：《江苏大学学报（医学版）》2022年第5期398-402,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81800431);武汉大学医学部教学研究项目(2018039)。

摘　　要：目的:制备小鼠深静脉血栓(deep venous thrombosis, DVT)实验动物模型。方法:56只C57BL/6J小鼠随机分成正常对照组、假手术组、手术1组、手术2组、内毒素+手术组。手术1组为结扎下腔静脉90%;假手术组除不结扎外,余操作同手术1组;内毒素+手术组和手术2组于术前2 h分别腹腔注射内毒素和等容量的生理盐水,余操作同手术1组。评估小鼠术前及下腔静脉狭窄术后一般情况,多普勒超声检测术前和术后即刻小鼠下腔静脉周围结构及血管图像,术后48 h取材测定血栓长度、重量和形成血栓例数百分比作为评估DVT模型的定量指标。结果:下腔静脉结扎术后总死亡率为25.0%,手术成功率为70.8%。术后即刻超声显示仍有静脉血流通过结扎点。正常对照组和假手术组未形成血栓,手术1组与手术2组血栓大小相比,差异无统计学意义(P>0.05)。与手术2组相比,内毒素+手术组血栓长度增加,差异有统计学意义(P<0.05),血栓重量增加,但差异无统计学意义(P>0.05)。结论:本实验制备的小鼠DVT模型具有良好的操作性、稳定性、有效性和实用性。Objective:To prepare the experimental animal model of deep vein thrombosis(DVT)in mice.Methods:Fifty-six C57BL/6J mice were randomly divided into five groups:normal control group,sham operation group,operation group 1,operation group 2 and endotoxin+operation group.In operation group 1,90%of inferior vena cava were ligated.The sham operation group performed the same operation except ligation.Endotoxin+operation group and operation group 2 were intraperitoneally injected with endotoxin and equal volume of normal saline 2 hours before surgery,and the other operations were the same as operation group 1.The general situation of the mice before and after the inferior vena cava stenosis was evaluated.The images of the structures and vessels around the inferior vena cava were obtained by Doppler ultrasonography before and immediately after the operation.The length,weight and percentage of thrombosis were determined 48 h after the operation as the quantitative indexes to evaluate the DVT model.Results:The total mortality after inferior vena cava stenosis was 25.0%and the success rate was 70.8%.Immediate postoperative ultrasound showed that there was still venous blood flow through the ligation site.No thrombus was observed in the normal control group and the sham operation group,and there was no significant difference in the size of thrombus between operation group 1 and operation group 2(P>0.05).Compared with the operation group 2,the length of thrombus in the endotoxin+operation group was significantly increased(P<0.05),and the weight of thrombus was increased,but the difference was not significant(P>0.05).Conclusion:The model of DVT in mice prepared in this study has good operability,validity and practicability.

关 键 词：深静脉血栓 下腔静脉 模型 小鼠 内毒素 

分 类 号：R543.6[医药卫生—心血管疾病]