Dicer在小鼠大脑星形胶质细胞静息态维持中的作用  

Dicer requiring for astrocyte quiescence in developing brains

作　　者：刘坤 陈婧菲 王寿玉 李涛 高兴 于彬 梅峰 肖岚 LIU Kun;CHEN Jing-fei;WANG Shou-yu;LI Tao;GAO Xing;YU Bin;MEI Feng;XIAO Lan(Department of Histology and Embryology,Chongqing Key Laboratory of Neurobiology,Brain and Intelligence Research Key Laboratory of Chongqing Education Commission,Army Medical University,Chongqing 400038,China;Department of Neurosurgery,Xinqiao Affiliated Hospital,Army Medical Universit,Chongqing 400038,China)

机构地区：[1]陆军军医大学组织学胚胎学教研室,脑与智能研究院,重庆400038 [2]陆军军医大学新桥医院神经外科,重庆400038

出　　处：《解剖学报》2022年第4期407-411,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(31970921)。

摘　　要：目的探讨微小RNA(miRNA)内切酶Dicer对于发育期小鼠中枢神经系统(CNS)中星形胶质细胞静息态维持的影响。方法构建hGFAP-CreERT;Dicer fl/fl;mT/mG和Dicer fl/fl;mT/mG转基因小鼠,在小鼠幼年时期用他莫昔芬(TMF)进行诱导,诱导完成后根据年龄及是否表达hGFAP-CreERT分别将小鼠分为7 d、10 d、14 d Dicer选择性敲除组与对照组,共6组,每组4只,共24只。用免疫荧光染色方法观察发育期小鼠CNS中星形胶质细胞的改变。结果选择性敲除星形胶质细胞Dicer后,CNS中星形胶质细胞与小胶质细胞均被明显激活。结论星形胶质细胞中的Dicer及miRNA在维持星形胶质细胞的静息状态中发挥了重要作用。Objective To explore whether microRNAs(miRNA)in astrocytes participate in regulating the phenotypic switch in the developing central nervous system(CNS).Methods The hGFAP-CreERT;Dicer fl/fl;mT/mG and Dicer fl/fl;mT/mG mice were generated and induced by tamoxifen(TMF).They were divided into 7 days,10 days,14 days Dicer conditional knock out(Dicer CKO)group and control group according to their age and the expression of hGFAP-CreERT.These 24 mice were divided into six groups and each group contained four mice.Then the change of astrocytes in the developing CNS was observed by immunofluorescenct staining.Results Both of the densities of astrocytes and microglial cells increased in the Dicer CKO mice brains as compared to littermate controls.Conclusion Dicer and miRNA of astrocytes are important for astrocytes quiescence in developing brains.

关 键 词：脑白质 星形胶质细胞 微小RNA 小胶质细胞 胶质纤维酸性蛋白 免疫荧光 小鼠 

分 类 号：Q189[生物学—神经生物学]