PA型紫苏不同部位8种酚酸和黄酮类成分的含量相关性研究  

Content correlation of eight phenolic acids and flavonoids in different medicinal parts of PA-type Perilla germplasms

作　　者：张琛武 郭佳琪 李卫萍[2] 落艳娇 姚宇 徐超群 申国安 索风梅[2] 郭宝林[2] ZHANG Chen-wu;GUO Jia-qi;LI Wei-ping;LUO Yan-jiao;YAO Yu;XU Chao-qun;SHEN Guo-an;SUO Feng-mei;GUO Bao-lin(Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China)

机构地区：[1]山西药科职业学院,山西太原030031 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中国中药杂志》2022年第13期3447-3451,共5页China Journal of Chinese Materia Medica

基　　金：国家中药标准化项目(ZYBZH-Y-JIN-34)。

摘　　要：该研究选取了10份PA型紫苏种质,检测叶、茎和果实3个不同部位,分别对应紫苏叶、紫苏梗和紫苏子3种药材中含量较高的2种酚酸[迷迭香酸(RA)和咖啡酸(CA)]以及6种黄酮[野黄芩素-7-O-二葡萄糖醛酸苷(SDG)、木犀草素-7-O-二葡萄糖醛酸苷(LDG)、芹菜素-7-O-二葡萄糖醛酸苷(ADG)、野黄芩素-7-O-葡萄糖醛酸苷(SG)、木犀草素-7-O-葡萄糖醛酸苷(LG)、芹菜素-7-O-葡萄糖醛酸苷(AG)]的含量。叶中酚酸总量为3.991~12.028 mg·g^(-1)、黄酮总量为12.309~25.071 mg·g^(-1),远远大于茎(分别为0.586~2.015、0.879~1.413 mg·g^(-1))和果实(分别为0.004~2.222、0.651~1.936 mg·g^(-1))。酚酸类成分中有5份果实中未能检测出RA,其余5份对应样品中紫苏叶与果实之间RA含量均呈现显著负相关。黄酮类成分茎中仅能检测出LG和LDG,果实中缺失SG和SDG,而其他几种黄酮也在部分样品中未能检出,叶和果实的总黄酮、LG含量呈现显著正相关,茎和果实的LG含量呈现显著正相关。10份茎样本中,有7份达到了《中国药典》(2020年版)规定的紫苏梗迷迭香酸不低于0.1%的标准。而仅1份果实达到药典规定的紫苏子迷迭香酸不低于0.25%的标准。In this study,10 PA-type Perilla germplasms were selected to detect the content of two phenolic acids,i.e.,rosmarinic acid(RA)and caffeic acid(CA),and six flavonoids,including scutellarin-7-O-diglucuronoside(SDG),luteolin-7-O-diglucuronoside(LDG),apigenin-7-O-diglucuronoside(ADG),scutellarin-7-O-glucuroside(SG),luteolin-7-O-glucuroside(LG),and apigenin-7-O-glucuroside(AG)in leaves,stems,and fruits.The total content of phenolic acids and flavonoids in leaves was 3.991-12.028 mg·g^(-1)and 12.309-25.071 mg·g^(-1),respectively,which was much higher than that in stems(0.586-2.015 mg·g^(-1)and 0.879-1.413 mg·g^(-1),respectively)and fruits(0.004-2.222 mg·g^(-1)and 0.651-1.936 mg·g^(-1),respectively).RA was detected in five fruit samples,and RA content between leaves and fruits showed a significant negative correlation in the other five samples.For flavonoids,only LG and LDG could be detected in stems,and SG and SDG were not detected in fruits,while other flavonoids were not detected in some samples.The content of total flavonoids and LG in leaves and fruits was significantly positively correlated,and the content of LG in stems and fruits was significantly positively correlated.In 10 stem samples,seven met the standard that the content of RA in the stem should be not less than 0.1%specified in the Chinese Pharmacopoeia(2020 edition).Only one fruit sample reached the standard of RA content in the fruit not less than 0.25%specified in the Chinese Pharmacopoeia.

关 键 词：PA型紫苏 叶 茎 果实 酚酸 黄酮 含量相关性 

分 类 号：R284.1[医药卫生—中药学]