诺丽种子化学成分及其α-葡萄糖苷酶抑制活性研究  

Chemical constituents withα-glucosidase inhibitory activities from seeds of Morinda citrifolia(Noni)

作　　者：徐盟 余章昕[2,3] 张斌 李小宝[2,3] 李牧原 徐伟[1] 陈光英 XU Meng;YU Zhang-xin;ZHANG Bin;LI Xiao-bao;LI Mu-yuan;XU Wei;CHEN Guang-ying(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou350122,China;Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University,Haikou 571158,China;Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province,College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]海南师范大学热带药用资源化学教育部重点实验室,海南海口571158 [3]海南师范大学化学化工学院海南省热带药用植物化学重点实验室,海南海口571158

出　　处：《中国中药杂志》2022年第13期3519-3525,共7页China Journal of Chinese Materia Medica

基　　金：海南省教育厅项目(Hnky2021-30);海南省重点研发项目(ZDYF2019116);教育部创新团队项目(IRT-16R19);海南省院士创新平台科研专项(YSPTZX202030)。

摘　　要：综合运用多种现代色谱分离方法对诺丽种子的化学成分及其α-葡萄糖苷酶抑制活性进行研究,从乙酸乙酯部位分离得到15个化合物。通过理化常数和波谱分析,并通过与文献对照,分别鉴定为(9S,2E,4Z,7Z)-9-羟基-2,4,7-葵三烯酸(1)、壬二酸(2)、东莨菪内酯(3)、熊果酸(4)、槲皮素(5)、环(L-亮-L-异亮)二肽(6)、环(L-苯丙-L-异亮)二肽(7)、环(L-苯丙-L-缬)二肽(8)、环(L-亮-L-缬)二肽(9)、环(L-苯丙-L-亮)二肽(10)、咖啡酸(11)、3,4-二羟基肉桂醛(12)、对羟基苯甲酸(13)、对羟基肉桂酸(14)、对羟基苯乙醇(15),其中,化合物1为新化合物,化合物7~10和12为首次从茜草科及巴戟天属中分离得到,除化合物3外,其余化合物均为首次从诺丽种子中分离得到。对所有化合物进行了α-葡萄糖苷酶抑制活性测试,化合物3~5具有一定的活性,其IC_(50)分别为160、133、120μmol·L^(-1)。The present study investigated the chemical constituents and inhibitory activities againstα-glucosidase from the seeds of Morinda citrifolia(Noni)by the chromatographic technique and semi-preparative HPLC.Fifteen compounds were isolated from the ethyl acetate extract of the seeds,and their structures were identified on the basis of physiochemical characteristics and spectroscopic data as(9S,2E,4Z,7Z)-9-hydroxydeca-2,4,7-trienoic acid(1),azelaic acid(2),scopoletin(3),ursolic acid(4),quercetin(5),cyclo-(L-Leu-L-Ile)(6),cyclo-(L-Phe-L-Ile)(7),cyclo-(L-Phe-L-Val)(8),cyclo-(L-Leu-L-Val)(9),cyclo-(L-Phe-L-Leu)(10),caffeic acid(11),3,4-dihydroxycinnamaldehyde(12),p-hydroxybenzoic acid(13),p-hydroxy-cinnamic acid(14),and p-hydroxyphenethyl alcohol(15).Among them,compound 1 was a new fatty acid and compounds 7-10 and 12 were isolated from Morinda plant in the Rubiaceae family for the first time.Compounds 1,2 and 4-15 were isolated from the seeds of M.citrifolia(Noni)for the first time.All isolated compounds were evaluated for the inhibitory activities againstα-glucosidase and compounds 3-5 showed potential inhibitory activity with IC_(50) values of 160,133,and 120μmol·L^(-1),respectively.

关 键 词：诺丽种子 脂肪酸 环二肽 香豆素 α-葡萄糖苷酶抑制活性 

分 类 号：R284.1[医药卫生—中药学]