高效液相色谱法同时测定化妆品中5种抗氧化成分  

Simultaneous determination of 5 antioxidant components in cosmetics by high performance liquid chromatography

作　　者：刘丹 何燕伶 邱红燕[1] 马莹[1] Dan Liu;Yanling He;Hongyan Qiu;Ying Ma(Guizhou Institute for Food and Drug Control,Guiyang,Guizhou 550004,China;ShenQi Ethnic Medicine College of Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]贵州省食品药品检验所,贵州贵阳550004 [2]贵州医科大学神奇民族医药学院,贵州贵阳550004

出　　处：《日用化学工业》2022年第8期858-862,共5页China Surfactant Detergent & Cosmetics

摘　　要：建立了高效液相色谱法同时测定化妆品中儿茶素、表儿茶素、没食子酸、芦丁、槲皮素含量的分析方法。待测物经CAPCELL PAK MG C_(18)色谱柱(4.6 mm×250 mm)分离,梯度洗脱;流动相为0.1%磷酸水溶液-甲醇,儿茶素、表儿茶素、没食子酸测定波长为280 nm,芦丁、槲皮素测定波长为360 nm,柱温为30℃,PDA检测器,流速1.0 mL/min。结果显示,儿茶素、表儿茶素、没食子酸、芦丁、槲皮素在0.5945~15.4036μg/mL范围内线性良好,r>0.999。上述5种化合物的检出限范围为0.0251~0.1284 ng/mL,24 h内稳定性良好。水基质中的平均回收率为99.7%,乳霜基质中的平均回收率为99.4%。该方法操作简便,准确性良好,适用于同时测定化妆品中儿茶素、表儿茶素、没食子酸、芦丁、槲皮素5种成分含量。This experiment adopts high performance liquid chromatography method to simultaneously detect the content of catechins,epicatechins,gallic acid,rutin and quercetin in cosmetics,and provides certain reference for the detection of antioxidant ingredients in plant extracts in cosmetics.Test samples were separated by CAPCELL PAK MG C_(18) column(4.6 mm×250 mm)and underwent gradient elution.The mobile phase was 0.1%aqueous phosphate solution-methanol,the wavelength of catechins,epicatechins,and gallic acid was 280 nm and that of rutin and quercetin was 360 nm.The column temperature was 30℃with the PDA detector,and the flow rate was 1.0 mL/min.The five compositions are linearly good in the range of 0.5945-15.4036μg/mL,and the r>0.999.The detection limit of this method is 0.0251-0.1284 ng/mL.The average recovery rate in the water matrix is 92%-113%,and that in the cream matrix is 90%-110%.This method is easy to operate,and has good stability.Therefore,it is accurate and suitable for simultaneously measuring the content of catechins,epicatechins,gallic acid,rutin and quercetin in cosmetics.

关 键 词：高效液相色谱法 化妆品 抗氧化 双波长 含量测定 

分 类 号：TQ658[化学工程—精细化工]