梅花鹿茸不同生长时期转录组及蛋白组联合分析  

Joint analysis of transcriptome and proteome of Cervus nippon Temminck antler in different periods

作　　者：慧芳 孙天霞 薛东明 姜英男 赵雨[1] HUI Fang;SUN Tianxia;XUE Dongming;JIANG Yingnan;ZHAO Yu(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学人参科学研究院,长春130117

出　　处：《吉林中医药》2022年第8期975-979,共5页Jilin Journal of Chinese Medicine

基　　金：国家重点研发计划(2018YFC1706603)。

摘　　要：目的了解鹿茸再生及快速生长过程中基因和蛋白的动态变化,阐明鹿茸生长发育机制。方法以梅花鹿(Cervus nippon Temminck)的鹿茸[初生期(PS)、快速生长期(RG)、骨化期(OS)鹿茸组织]作为实验材料,基于Illumina Hiseq和iTRAQ技术在转录和蛋白水平进行分析,根据差异基因和差异蛋白结果筛选与鹿茸再生和快速生长有关的基因和蛋白。结果2组分别得到11294(PSvsRG)和4924(PSvsOS)个显著差异基因(DEGs),2组的蛋白组数据显示,分别得到236、211个差异蛋白(DAPs),相关性分析显示转录组和蛋白组数据相关性分析呈弱相关。在2组中分别有54、51个DEGs编码了其相应的DAPs,其中,在2组中分别得到35和20个在转录组和蛋白组中调节方向相同的DEGs和相应的DAPs。对候选基因/蛋白进行KEGG富集分析,分别富集到10和12个信号通路。其中,2组共同富集到的通路包括:血管平滑肌收缩、p53信号通路、黏着斑、蛋白质消化吸收、TGF-β信号通路、ECM-受体相互作用信号通路。只富集到快速生长期的信号通路包括:戊糖磷酸途径、糖酵解/糖异生、果糖和甘露糖代谢及代谢途径。只富集到骨化期的信号通路包括:破骨细胞分化、肌动蛋白细胞骨架的调节、紧密连接、RNA转运、mRNA监测途径信号通路。共有10个候选基因/蛋白(CALD1、THBS2S、COL9A、ALDO、COL1A、HSPG2、SIRPA、CSRP、MYH9、H1-5)注释到上述信号通路。结论在鹿茸生长发育期间许多基因和蛋白质在鹿茸快速生长期和骨化期表现出不同的丰度。转录组和蛋白组数据相关性较差。筛选出与鹿茸生长发育相关的蛋白为:CALD1、THBS2S、COL9A、ALDO、COL1A、HSPG2、SIRPA、CSRP、MYH9、H1-5以上结果为阐明鹿茸再生的分子机制提供了重要的理论依据。Objective To understand the dynamic changes of genes and proteins in the process of antler regeneration and rapid growth,and to clarify the mechanism of antler growth and development.Methods The antler tissues of Cervus nippon Temminck in the primary stage (PS),the rapid growth stage (RG) and the ossification stage (OS) were analyzed as experimental materials at the transcription and protein levels based on the Illumina Hiseq and iTRAQ techniques.The genes and proteins related to the deer antler regeneration and rapid growth were screened according to the results of differential genes and differential proteins.Results The two groups obtained 11 294 (PS vs RG) and 4 924 (PS vs OS) differentially expressed genes (DEGs),respectively.The proteome data of the two groups showed that 236 and 211 differential abundance proteins (DAPs) were obtained,respectively.A correlation analysis showed that the transcriptome and proteome data were weakly correlated.In the two groups,54 and 51 DEGs encoded their corresponding DAPs,respectively,among which 35 and 20 DEGs and corresponding DAPs with the same regulatory direction in the transcriptome and proteome were obtained in the two groups,respectively.A KEGG enrichment analysis was performed on candidate genes/proteins,and 10 and 12 signaling pathways were enriched,respectively.Among them,the pathways commonly enriched in the two groups included:vascular smooth muscle contraction,p53 signaling pathway,focal adhesion,protein digestion and absorption,TGF-β signaling pathway,and ECM-receptor interaction signaling pathway.Signaling pathways enriched only in the rapid growth stage included:pentose phosphate pathway,glycolysis/gluconeogenesis,fructose and mannose metabolism and metabolic pathways.Signaling pathways enriched only in the ossification stage included:osteoclast differentiation,regulation of the actin cytoskeleton,tight junctions,RNA transport,and mRNA surveillance pathways.A total of 10 candidate genes/proteins (CALD1,THBS2S,COL9A,ALDO,COL1A,HSPG2,SIRPA,CSRP,MYH9,H1-5) wer

关 键 词：梅花鹿 转录组测序 蛋白组 iTRAQ定量分析技术 鹿茸再生 

分 类 号：R285.5[医药卫生—中药学]