样本状态对血浆EB病毒DNA检测结果准确性的影响  

Influence of specimen state on the accuracy of plasma Epstein-Barr virus DNA determination results

作　　者：王筱雨[1] 色米热·艾尼瓦尔 李士军[1] WANG Xiaoyu;SEMIRE Ainiwaer;LI Shijun(Department of Clinical Laboratory,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,Liaoning,China;Department of Clinical Laboratory,Shache County People's Hospital Xinjiang Uygur Autonomous Region,Kashgar 844700,Xinjiang,China)

机构地区：[1]大连医科大学附属第一医院检验科,辽宁大连116011 [2]新疆维吾尔自治区莎车县人民医院检验科,新疆喀什844700

出　　处：《检验医学》2022年第7期615-617,共3页Laboratory Medicine

摘　　要：目的探讨EB病毒(EBV)DNA在血浆样本溶血和脂血状态下的表达差异。方法收集40例EBV DNA阳性样本,随机分为2组,每组20例。将EBV DNA阳性样本与灭菌蒸馏水按照1∶1比例混合,作为对照样本,采用荧光定量聚合酶链反应(PCR)检测其在模拟溶血(血红蛋白浓度分别为60.50、30.25、15.13 g/L)及模拟脂血(三酰甘油浓度分别为6.36、4.24、2.12 mmol/L)状态下EBV DNA含量,并进行统计学分析。结果血红蛋白浓度为60.50和30.25 g/L时,EBV DNA阳性血浆溶血样本和对照样本的PCR检测结果差异有统计学意义(P<0.05);血红蛋白浓度为15.13 g/L时,EBV DNA阳性血浆溶血样本和对照样本的PCR检测结果差异无统计学意义(P>0.05)。EBV DNA阳性血浆各浓度脂血样本和对照样本的PCR检测结果差异均无统计学意义(P>0.05)。结论血红蛋白≤15.13 g/L的溶血样本和三酰甘油≤6.36 mmol/L的脂血样本对EBV DNA检测结果无影响。Objective To investigate the expression of Epstein-Barr virus(EBV)DNA in hemolysis and lipoidemia of plasma samples.Methods A total of 40 plasma positive samples with confirmed EBV DNA were collected and divided into 2 groups randomly(20 cases for each group).The EBV DNA positive samples were mixed with sterilized distilled water in a 1∶1 ratio as control samples.The levels of EBV DNA in simulated hemolysis(hemoglobin concentration 60.50,30.25 and 15.13 g/L)and simulated lipoidemia(triglyceride concentration 6.36,4.24 and 2.12 mmol/L)were determined by fluorescence quantitative polymerase chain reaction(PCR),and statistical analysis was performed.Results When the hemoglobin concentrations were 60.50 and 30.25 g/L,the PCR results of EBV DNA positive plasma hemolysis samples and control samples showed statistical significance(P<0.05).When the hemoglobin concentration was 15.13 g/L,there was no statistical significance in the PCR results between EBV DNA positive plasma hemolysis samples and control samples(P>0.05).There was no statistical significance in the PCR results between lipoidemia samples and control samples(P>0.05).Conclusions Hemolysis samples with hemoglobin concentration≤15.13 g/L and lipoidemia samples with triglyceride concentration≤6.36 mmol/L had no effect on the determination of EBV DNA plasma samples.

关 键 词：EB病毒DNA 荧光定量聚合酶链反应 溶血 脂血 

分 类 号：R373.1[医药卫生—病原生物学]