96个大白菜品种根肿病抗性鉴定及位点检测  

Identification and detection of clubroot resistance loci of 96 Chinese cabbage cultivars resistance to Plasmodiophora brassicae

作　　者：魏小春[1,2] 原玉香 赵艳艳[1] 杨双娟[1] 王志勇[1] 苏贺楠[1] 李军党 李林 牛刘静 张晓伟 WEI Xiaochun;YUAN Yuxiang;ZHAO Yanyan;YANG Shuangjuan;WANG Zhiyong;SU Henan;LI Jundang;LI Lin;NIU Liujing;ZHANG Xiaowei(Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;School of Agricultural Science,Zhengzhou University,Zhengzhou 450001,Henan,China)

机构地区：[1]河南省农业科学院园艺研究所,郑州450002 [2]郑州大学农学院,郑州450001

出　　处：《中国瓜菜》2022年第8期27-34,共8页China Cucurbits And Vegetables

基　　金：国家自然科学基金(31801874);河南省农业科学院杰出青年基金(2021JQ03);河南省农业科学院科技创新团队(2022TD06)。

摘　　要：为了明确大白菜抗根肿病基因类型和品种抗性特征之间的关系,利用同为4号生理小种的新野老菌和新野新菌,采用灌根法对96个大白菜品种进行抗病性鉴定,并利用CRa、CRb、CRc、Crr1、Crr2、Crr3等6个抗根肿病位点标记检测不同品种所含有的抗性位点。结果表明,农科一号、山地王1号、春美一号等31个品种对新野老菌和新野新菌均表现出抗性,且这些材料大多含有CRa和CRb抗病位点,个别品种含有Crr1、Crr2或Crr3抗病位点。只含有Crr2和CRb抗病位点的大白菜品种如Chiifu、德高荣耀、吉红娃娃、玲珑二号对新野老菌或新菌却表现出感病症状,笔者推测Crr2和CRb可能不是主要的抗病位点。此外,笔者发现只含有CRb位点的山地王2号B型对新野老菌和新菌均表现出抗性,说明该品种可能含有新的抗病基因。研究结果为大白菜抗根肿病种质资源鉴定及新品种选育奠定了基础。In order to clarify the relationship between resistance gene types and resistance characteristics of Chinese cabbage cultivars,96 Chinese cabbage cultivars were identified for resistance by root dipping method using new and old isolates of Xinye Plasmodiophora brassicaeis race 4 and six resistance loci including CRa,CRb,CRc,Crr1,Crr2 and Crr3 were used to detect the resistance loci of different varieties.The results showed that,31 varieties,including Nongke 1,Shandiwang 1 and Chunmei 1,showed resistance to both new and old fungus Xinye isolates,and most of these varieties had CRa and CRb resistance genes,while some varieties had Crr1,Crr2,or Crr3 resistance genes.However,Chinese cabbage varieties having only Crr2 and CRb resistance loci,such as Chiifu,Degaorongyao,Jihongwawa and Linglong 2,showed susceptible symptoms to new or old Xinye isolates.we speculated that Crr2 and CRb may not be the main resistance loci.In addition,we found that Shandiwang 2 typeB having only CRb loci showed resistance to both new and old Xinye isolates,indicating that there may be new resistance genes in Shandiwang 2 typeB.The results laid a foundation for the identification of germplasm resources and breeding of Chinese cabbage resistant to the disease.

关 键 词：大白菜 根肿病 抗性基因 生理小种 

分 类 号：S634.1[农业科学—蔬菜学]