LncRNA H19通过海绵miR-675和上调CDH13的表达促进肺癌的增殖、侵袭和上皮-间质转化  

LncRNA H19 promoted the proliferation,invasion and EMT of lung cancer through sponge miR-675 and up-regulation of CDH13 expression

作　　者：杜伟 王山水 张焕焕 DU Wei;WANG Shanshui;ZHANG Huanhuan(Department of Respiratory Medicine,Medical College of Qingdao University,Qingdao 266071,Shandong,China;Department of ICU,Gaotang People's Hospital Affiliated to Jining Medical College,Liaocheng 252800,Shandong,China;Department of Respiratory Medicine,Gaotang People's Hospital Affiliated to Jining Medical College,Liaocheng 252800,Shandong,China)

机构地区：[1]青岛大学附属医院呼吸内科,山东青岛266071 [2]济宁医学院附属高唐县人民医院ICU,山东聊城252800 [3]济宁医学院附属高唐县人民医院呼吸内科,山东聊城252800

出　　处：《西部医学》2022年第8期1121-1127,1132,共8页Medical Journal of West China

基　　金：国家自然科学基金项目(81703033);山东省医药卫生科技发展计划项目(2018WS008)。

摘　　要：目的探讨LncRNA H19对肺癌SPC-A1细胞增殖、侵袭和上皮-间质转化(EMT)的影响及其作用机制。方法实时荧光定量PCR检测LncRNA H19、miR-675和CDH13在SPC-A1细胞、BEAS-2B细胞中的差异表达;siRNA H19转染SPC-A1细胞,通过MTT、Transwell及Western blot检测SPC-A1细胞的增殖、侵袭和EMT能力。miRanda软件和双荧光素酶报告基因实验分析LncRNA H19和miR-675之间的作用靶点和相关性。miR-675 inhibitor转染SPC-A1细胞,检测SPC-A1细胞的增殖、侵袭和EMT能力。检测上调LncRNA H19通过miR-675对SPC-A1细胞增殖、侵袭和EMT的影响。TargetScan软件和双荧光素酶报告基因实验分析miR-675和CDH13之间的作用靶点和相关性。siRNA CDH13转染SPC-A1细胞,检测SPC-A1细胞的增殖、侵袭和EMT能力。RT-qPCR和Western blot检测LncRNA H19通过miR-675对CDH13表达的影响。结果与BEAS-2B细胞相比,SPC-A1细胞中LncRNA H19和CDH13表达上调,miR-675表达下调(P<0.05)。siRNA H19转染SPC-A1细胞明显抑制了细胞的增殖、侵袭与EMT;LncRNA H19靶向且负调控miR-675;上调LncRNA H19通过miR-675表达促进SPC-A1细胞恶性发展。miR-675靶向且负调控CDH13。siRNA CDH13转染SPC-A1细胞明显抑制了细胞的增殖、侵袭与EMT。LncRNA H19通过miR-675上调CDH13表达。结论LncRNA H19通过海绵miR-675和上调CDH13表达促进了SPC-A1细胞增殖、侵袭和EMT。Objective To investigate the effects of lncRNA H19 on proliferation,invasion and EMT of lung cancer SPC-A1 cells and its mechanism.Methods The differential expressions of LncRNA H19,miR-675 and CDH13 in SPC-A1 cells and BEAS-2B cells were detected by real-time quantitative PCR.SPC-A1 cells were transfected with siRNA H19,and the proliferation,invasion and EMT ability of SPC-A1 cells were detected by MTT,Transwell and Western blot assay.The target and correlation between LncRNA H19 and miR-675 were analyzed by miRanda and dual luciferase reporter gene assay.SPC-A1 cells were transfected with miR-675 inhibitor to detect the proliferation,invasion and EMT ability of SPC-A1 cells.The effects of upregulated LncRNA H19 on proliferation,invasion and EMT of SPC-A1 cells via miR-675 were detected.TargetScan and dual luciferase reporter assay were used to analyze the target and correlation between miR-675 and CDH13.SPC-A1 cells were transfected with siRNA CDH13 to detect the proliferation,invasion and EMT ability of SPC-A1 cells.The effect of LncRNA H19 on CDH13 expression through miR-675 was detected by RT-qPCR and Western blot.Results Compared to BEAS-2B cells,the expressions of LncRNA H19 and CDH13 were up-regulated(P<0.01),the expression of miR-675 was down-regulated(P<0.01)in SPC-A1 cells.The proliferation,invasion and EMT of SPC-A1 cells were significantly inhibited by siRNA H19 transfection.LncRNA H19 targeted and negatively regulated miR-675.Upregulation of LncRNA H19 through miR-675 or down-regulation of miR-675 expression promoted the malignant development of SPC-A1 cells.miR-675 targeted and negatively regulated CDH13.The proliferation,invasion and EMT of SPC-A1 cells were significantly inhibited by siRNA CDH13 transfection.LncRNA H19 up-regulated the expression of CDH13 by miR-675.Conclusion LncRNA H19 promoted proliferation,invasion and EMT of SPC-A1 cells through spongy miR-675 and up-regulation of CDH13 expression.

关 键 词：Lnc RNA H19 miR-675 CDH13 SPC-A1细胞 增殖 侵袭 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]