独活寄生汤调节淋巴系统防治类风湿关节炎  

DuhuoJisheng Decoction Regulating Lymphatic System to Prevent Rheumatoid Arthritis

作　　者：郑为超[1] 王岚 方叶 尤佳锋[1] 梁倩倩 ZHENG Weichao;WANG Lan;FANG Ye;YOU Jiafeng;LIANG Qianqian(School of Medicine,Anhui University of Science and Technology,Huainan Anhui 232001,China;Spinal Disease Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Longhua Hospital affiliated to Shanghai University of Chinese Medicine 200032,China)

机构地区：[1]安徽理工大学医学院,安徽淮南232001 [2]上海市中医药研究院脊柱病研究所,上海200032 [3]上海中医药大学附属龙华医院,上海200032

出　　处：《安徽理工大学学报（自然科学版）》2022年第2期95-101,共7页Journal of Anhui University of Science and Technology:Natural Science

基　　金：国家自然科学基金委员会优秀青年科学基金资助项目(81822050);上海市曙光计划项目(19SG39)。

摘　　要：目的探讨独活寄生汤(DHJST)对类风湿关节炎模型小鼠淋巴系统及免疫反应的影响。方法采用随机数字表法将10只3月龄TNF-α转基因(TNF-Tg)小鼠随机分为模型组(TNF-Tg)和DHJST组(5只/组);5只野生型同窝小鼠作为正常对照。DHJST组采用独活寄生汤灌胃,模型组和正常对照组(Control)采用相同体积的生理盐水灌胃,1次/d,连续给药12周后取材。采用阿尔新蓝-苏木精(ABOG)染色观察骨量及滑膜炎症情况;免疫荧光染色,观察促炎M1型巨噬细胞、淋巴管新生以及分布情况。结果与模型组比较,DHJST组小鼠软骨侵蚀、骨侵蚀以及滑膜炎症情况明显改善(P<0.05),促炎M1型巨噬细胞数量减少(P<0.01),毛细淋巴管生成增加(P<0.01),集合淋巴管分布广(P<0.01)。结论DHJST可通过调控淋巴系统,改善小鼠关节炎症情况,进而减缓类风湿关节炎进展。Object To explore the effects of DuhuoJisheng Decoction on the lymphatic system and immune response of mice with rheumatoid arthritis.Method 15 mice were used in this experiment,of which 103-month-old TNF-αtransgenic(TNF-Tg)mice were randomly divided into model group and DHJST group,and another 5 wild-type littermates were taken as negative controls.DHJST group mice were treated with Duhuo Jisheng Decoction.In the control and model group,the same volume of normal saline was intragastrically administered.All mice were dosed once a day for 12 weeks.ABOG(Alcian Blue-Hematoxylin)staining is used to observe bone mass and synovial inflammation.And through the IF(immunofluorescence staining)method,the distribution was explored of M1 macrophages that promote inflammation and the situation of lymphangiogenesis.Results After the administration of Duhuojijietang,the articular cartilage erosion,bone erosion and synovial inflammation of TNF-Tg mice were significantly improved(P<0.05).Immunostaining showed that the number of M1 macrophages decreased and the immune response was weakened(P<0.05)and the number of lymphatic capillaries and the distribution area of lymphatic collecting was increased(P<0.05).Conclusion DHJST can not only improve the degree of joint inflammation in TNF-Tg mice,but also promote the reflux function of lymphatic vessels,thereby reducing inflammation.

关 键 词：独活寄生汤 类风湿关节炎 TNF-Tg转基因鼠 巨噬细胞 淋巴管 

分 类 号：R392.9[医药卫生—免疫学]