小花山柰多糖的提取表征及对IEC-6细胞增殖、迁移的作用  

Extraction and Characterization of Polysaccharides from Kaempferiae Parviflorae Rhizoma and its Biological Functions on Proliferation and Migration in the Model of IEC-6 Cell

作　　者：孙加燕 朱金燕[1] 陈雪晴 SUN Jia-yan;ZHU Jin-yan;CHEN Xue-qing(Department of Pharmacy,Anqing Medical College,Anqing 246052,China)

机构地区：[1]安庆医药高等专科学校药学系,安徽安庆246052

出　　处：《宜春学院学报》2022年第6期28-33,共6页Journal of Yichun University

基　　金：安徽省高校自然科学研究重点项目(编号:KJ2021A1294)。

摘　　要：目的:提取分离小花山柰多糖,初步研究小花山柰多糖对IEC-6细胞增殖和迁移的影响。方法:采用水提醇沉法提取小花山柰粗多糖;采用DEAE-52纤维素柱及Superdex-200凝胶过滤柱对粗多糖进行分离纯化得到小花山柰精制多糖(Xps),并对精制糖的主要成分包括多糖含量、蛋白质、糖醛酸进行含量分析;采用HPGPC、FT-IR、SEM、HPLC等方法分别对多糖的分子量、官能团、微观结构、单糖组成进行分析;采用CCK-8法、细胞划痕法初步研究小花山柰多糖对IEC-6细胞增殖、迁移的影响。结果:获得一种小花山柰精制多糖(Xps),该精制糖的单糖组成有四种,分别是鼠李糖、半乳糖醛酸、葡萄糖、半乳糖。精制糖的分子量(Mw)是29.94 kDa,且该精制糖在10 ug/mL~100 ug/mL时能够显著促进IEC-6细胞增殖、迁移。结论:小花山柰精制多糖(Xps),属于高分子酸性杂多糖,一定浓度小花山柰精制多糖(Xps),能够促进IEC-6细胞增殖和迁移,提示小花山柰精制多糖(Xps),有利于受伤的大鼠肠黏膜机械屏障的重构。Objective:This study aims to obtain water-soluble polysaccharide(Xps) that is isolated and purfied from Kaempferiae Parviflorae Rhizoma and to investigate its biological functions on proliferation and migration in the model of IEC-6 cells.Methods:The crude polysaccharides(Xps) were extracted from Kaempferiae Parviflorae Rhizoma by water extraction and alcohol precipitation.Then it was separated and purified by DEAE-52 column and Superdex 200 column.Its main components,including polysaccharide,protein and uronic acid were analyzed.The molecular weight,functional group,microstructure and monosaccharide composition of Xps were analyzed by HPGPC,FT-IR,SEM and HPLC,respectively.The effects of Xps on IEC-6 cell proliferation and migration was studied by CCK-8 and cell migration experiments.Results:The Xps with molecular weight(Mw) of 29.94 kDa were obtained and composed of rhamnose,galacturonic acid,glucose and galactose.It significantly promoted the proliferation and migration of IEC-6 cells at the concentration of 10-100 ug/mL.Conclusion:A certain concentration of Xps could promote the proliferation and migration of IEC-6 cells,suggesting that it is beneficial to the reconstruction of intestinal mucosal mechanical barrier in injured rats.

关 键 词：小花山奈 多糖 结构表征 增殖 迁移 

分 类 号：R944.5[医药卫生—药剂学]