富血小板血浆对关节软骨再生作用的实验研究  

Experimental Study on the Effect of Platelet-rich Plasma on Articular Cartilage Regeneration

作　　者：王阿利塔 丁国良 贺超 邰图力格勒 戎英军[2] 慈昕元 张谦[3] 黄宇奇 何浩川 WANG Alita;DING Guoliang;HE Chao;Taituligele;RONG Yingjun;CI Xinyuan;ZHANG Qian;HUANG Yuqi;HE Haochuan(Baotou Medical College,Baotou014040 China;Baotou Central Hospital,Baotou014040 China;Inner Mongolia Medical University,Hohhot 010110 China)

机构地区：[1]包头医学院,内蒙古包头014040 [2]包头市中心医院,内蒙古包头014040 [3]内蒙古医科大学,呼和浩特010110

出　　处：《内蒙古医学杂志》2022年第6期660-662,F0004,共4页Inner Mongolia Medical Journal

基　　金：包头医学院研究生科研创新计划资助项目(编号:bycx2020010);内蒙古医科大学科技百万工程联合项目[编号:YKD2018KJBW(LH)085]。

摘　　要：目的研究富血小板血浆(platelet-rich plasm,PRP)对关节软骨的再生作用。方法32只SD(Sprague-Dawley)大鼠,随机分为正常组(CK)、假手术组(Sham)、关节炎组(OA)及PRP组,每组8只。正常组不做任何处置;假手术组切开膝关节腔;关节炎组改良Hulth法制造关节炎模型;PRP组改良Hulth法造模后关节腔内注射PRP。6周后观察软骨大体形态并进行Pelletier评分,电镜下观察软骨细胞改变,用免疫组化的方法检测软骨中IL1β、TNF-α表达的差异,统计学方法对比分析。结果大体观察Pelletier评分,关节炎组与正常组对比,统计学比较有显著差异(P<0.05);PRP组与关节炎组对比,统计学比较有显著差异(P<0.05)。电镜下观察软骨细胞超微结构,正常组、假手术组及PRP组基本正常,关节炎组软骨细胞明显异常。IL-1β、TNF-α在软骨中表达,PRP组较关节炎组显著降低,统计学比较有显著差异(P<0.05);关节炎组较正常组显著升高,统计学比较有显著差异(P<0.05)。结论PRP可以有效促进关节软骨的再生。Objective To study the regenerative effect of platelet-rich plasma(PRP)on articular car-tilage.Methods A total of 32SD(Sprague-Dawley)rats were randomized into normal,sham,arthritis and PRP group.The normal group will not do any disposal.The knee cavity was cut open in the sham group.Arthritis group A modified Hulth method to make the arthritis model.PRP group injected PRP after modified Hulth method.After 6 weeks,the gross morphology of cartilage was observed and Pelletier scored,chondrocyte changes were observed under electron microscope,and the differences in IL-1βand TNF-αin cartilage were detected by immunohistochemistry and analyzed by comparison.Results General observation and Pelletier score,Comparing the arthritis group with the normal group,there is a significant difference(P<0.05).The PRP group and the arthritis group in comparison,there was a significant differ-ence(P<0.05).Chondrocyte ultrastructure was observed by electron microscopy,while the normal group,sham group and PRP groups were basically normal,and the chondrocytes were significantly abnormal in the arthritis group.IL-1β,TNF-αexpressed in cartilage,The PRP group was significantly lower com-pared with the arthritis group,there was a significant difference(P<0.05);The arthritis group was signifi-cantly higher compared to the normal group,there was a significant difference(P<0.05).Conclusion PRP can effectively promote the regeneration of articular cartilage.

关 键 词：富血小板血浆 骨性关节炎 软骨 

分 类 号：R684[医药卫生—骨科学]