毒龙丹中马钱子炮制前后主要成分含量变化的UPLC法分析  

UPLC Analysis of Content Changes of Main Components of Strychnos nuxvornica L in Dulongdan Before and After Processing

作　　者：马鹏 张远哲 黄宁川 MA Peng;ZHANG Yuan-zhe;HUANG Ning-chuan(The First Affiliated Hospital of Guizhou University of traditional Chinese Medicine,Guizhou Guiyang 550000;College of Basic Medicine,Guizhou University of TCM,Guizhou Guiyang 550025,China)

机构地区：[1]贵州中医药大学第一附属医院,贵州贵阳550000 [2]贵州中医药大学基础医学院,贵州贵阳550025

出　　处：《广州化工》2022年第15期125-127,共3页GuangZhou Chemical Industry

摘　　要：建立测定毒龙丹中马钱子碱和士的宁含量的方法。采用UPLC法测定毒龙丹中马钱子碱和士的宁的含量。色谱柱为Agilent ZORBAX SB-C_(18)(2.1 mm×100 mm,1.8μm),乙腈(A)纯水(B)(0.2%的甲酸溶液)梯度洗脱,检测波长为260 nm,流速1.0 mL·min^(-1),柱温30℃,进样量为10μL。马钱子碱和士的宁的进样量分别10.36~82.88μg和12.79~102.32μg线性关系良好(r=0.9999),平均回收率为99.63%(RSD值为0.3%)和99.85%(RSD值为0.5%)。该方法简便、快速、重复性好,可作为毒龙丹中马钱子碱和士的宁的含量测定方法。A method for the determination of Strychnos nuxvornica L.in dulongdan was established.The contents of brucine and strychnine in dulongdan were determined by UPLC.The chromatographic column was Agilent Zorbax SB-C_(18)(2.1 mm×100 mm,1.8μm),acetonitrile(A)-pure water(B)(0.2%formic acid solution)gradient elution,detection wavelength was 260 nm,flow rate was 1.0 ml·min^(-1),column temperature was 30℃,injection volume was 10μL.The injection amounts of brucine and strychnine respectively were 10.36~82.88μg and 12.79~102.32μg,which had a good linear relationship(r=0.9999),and the average recoveries were 99.63%(RSD=0.3%)and 99.85%(RSD=0.5%).The method is simple,rapid and reproducible,and can be used for the determination of brucine and strychnine in dulongdan.

关 键 词：毒龙丹 超高压液相色谱法 马钱子碱 士的宁 含量分析 

分 类 号：R917[医药卫生—药物分析学]