哺乳动物配子冷冻保存并应用于珍稀濒危动物保护的技术策略  

Mammalian Gametes Cryopreserved and Applied to Technical Strategies for the Protection of Rare and Endangered Animals

作　　者：高峰 何琪富 吴盛辉 王少文 徐雪瑞 康健[1,2,3] 张涌 权富生[1,2,3] GAO Feng;HE Qifu;WU Shenghui;WANG Shaowen;XU Xuerui;KANG Jian;ZHANG Yong;QUAN Fusheng(College of Veterinary Medicine,Northwest A&F University,Yangling712100,China;Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs,Yangling712100,China;Shaanxi Animal Embryo Engineering Technology Research Center,Yangling712100,China)

机构地区：[1]西北农林科技大学动物医学院,杨凌712100 [2]农业农村部动物生物技术重点实验室,杨凌712100 [3]陕西省动物胚胎工程技术研究中心,杨凌712100

出　　处：《畜牧兽医学报》2022年第8期2479-2489,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31873033)。

摘　　要：配子(精子和卵子)冷冻保存是将配子在超低温条件下冷冻,可进行优秀家畜个体和珍稀濒危动物种质资源的长期保存。配子冷冻是珍稀濒危动物种质资源保护、保存和利用的一项重要内容,也是动物种质资源库建设的一项关键技术。本文对国内外精子和卵母细胞冷冻保存利用技术的研究现状进行了详细论述,同时介绍了配子冷冻-解冻后的损伤评估研究,提出了未来我国在濒危珍稀动物配子冷冻保存方面的应用策略和保种建议,以期为我国家畜生物种质资源库建设、家畜种质资源保护和开发利用以及珍稀濒危动物保护提供参考。The cryopreservation of gametes(sperm and oocytes)is the freezing of gametes under ultra-low temperature conditions,which can be used for long-term preservation of excellent individual livestock and rare and endangered animal germplasm resources.Gamete freezing is an important part of the protection,preservation and utilization of rare and endangered animal germplasm resources,and it is also a key technology for the construction of animal germplasm resources.This article discusses in detail the current research status of domestic and foreign sperm and oocyte cryopreservation and utilization.At the same time,it introduces the research on the damage assessment of gametes after freezing and thawing.The application strategies and recommendations for preservation of gametes of endangered and rare animals are expected to providereferences for the construction of my country’s livestock biological germplasm resource bank,the protection and development and utilization of livestock germplasm resources,and the protection of rare and endangered animals.

关 键 词：动物配子 精子冷冻 卵子冷冻 动物保种 珍稀濒危动物保护 

分 类 号：S814[农业科学—畜牧学] Q954.43[农业科学—畜牧兽医]