一株鹅星状病毒变异毒株的基因组特征与致病性分析  

Genomic Characteristics and Pathogenicity Analysis of One Variant Strain of Goose Astrovirus

作　　者：吕炫 贾北平 祝萌 于胜祖 张淼 王蓓[1] 朱英奇[1] 张云凯 王晴 王桂军[1] Lü Xuan;JIA Beiping;ZHU Meng;YU Shengzu;ZHANG Miao;WANG Bei;ZHU Yingqi;ZHANG Yunkai;WANG Qing;WANG Guijun(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;Anhui Wanxi White Goose Original Breeding Farm Co.Ltd.,Lu’an237158,China)

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽省皖西白鹅原种场有限公司,六安237158

出　　处：《畜牧兽医学报》2022年第8期2812-2818,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：安徽省科技重大专项“旱养模式水禽病毒性疫病防控技术研究与应用”(202003a06020012);安徽省家禽产业技术体系资助项目(2016-2020)。

摘　　要：旨在研究引起雏鹅痛风的新型鹅星状病毒流行株特征。本试验从安徽省鹅星状病毒阳性雏鹅组织中进行病毒分离,并对分离的毒株进行全基因组测序,随后进行遗传进化分析。结果表明,病毒液接种9~11日龄鹅胚可成功分离到病毒,将其命名为GASTV-ZM株。IFA(indirect immunofluorescence assay)鉴定为阳性。基因组序列分析表明,ZM株鹅星状病毒基因组全长7175nt,ORF2基因核苷酸和氨基酸与2014—2020年间分离毒株的同源性分别为96.7%~98.9%和96.9%~99.0%,ORF2基因编码的氨基酸序列结果显示,3个氨基酸突变位点与以往存在显著差异,ZM株与2016年以来在我国流行的新型鹅星状病毒处于同一进化分支,与2020分离株HNSQ-6株和XT1株同源性较高。本研究为进一步研究该病毒的致病性和致病机理奠定基础。The aim of this experiment was to study the characteristics of a novel goose astrovirus strain causing gosling gout.In this experiment,the virus was isolated from goose astrovirus positive goslings in Anhui Province,and the whole genome of the isolated virus strain was sequenced,followed by genetic evolution analysis.The results showed that the virus which named as GASTV-ZM strain was successfully isolated from goose embryos at 9-11days of age after inoculation,and was identified as postive by indirect immunofluorescence assay(IFA).The length of the genome of ZM strain was 7175nt.The nucleotide and amino acid homology of ORF2gene were 96.7%-98.9%and 96.9%-99.0%with the strains isolated from 2014to 2020,respectively.And the ORF2-encoded amino acid sequence showed that the three amino acid mutation sites were significantly different from the previous ones.ZM strain is in the same evolutionary branch with the novel goose astrovirus that has been circulating in China since 2016,and its homology with HNSQ-6strain and XT1strain isolated in 2020is high.This study laid a foundation for further study of the pathogenicity and pathogenicity mechanism of this virus.

关 键 词：新型鹅星状病毒ZM株 全基因测序 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]