Structural and functional characteristics of microbiota in oropharynx of sub-healthy children with gastrointestinal heat retention syndrome differentiated by traditional Chinese medicine  

作　　者：Jianhua Zhen He Yu Xiaofei Li Fei Dong Zi'an Zheng Xueyan Ma Yuxiang Wan Tiegang Liu Lu Fan Xiaohong Gu 

机构地区：[1]School of Life Sciences,Beijing University of Chinese Medicine,Beijing,102488,China [2]School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing,102488,China [3]Department of Traditional Chinese Medicine,People's Hospital of Laoling County,Laoling,253600,China [4]Institute of Chinese Medicine Epidemic Disease,Beijing University of Chinese Medicine,Beijing,102488,China [5]School of Basic Medicine,Guizhou University of Traditional Chinese Medicine,Guiyang,550025,China [6]Department of Acupuncture and Mini-invasive Oncology,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing,100029,China [7]Student Affairs Department,Beijing University of Chinese Medicine,Beijing,102488,China

出　　处：《Journal of Traditional Chinese Medical Sciences》2022年第3期230-236,共7页中医科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(81373769);the Scientific Research and Postgraduate Training Co-construction Project of Beijing Municipal Education Commission(1000062520115);the Province Natural Science Foundation of Beijing(7172131)。

摘　　要：Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHR

关 键 词：SUB-HEALTH Gastrointestinal heat retention syndrome Traditional Chinese medicine Microbiota in oropharynx 16S rDNA sequencing Children Differential genus Biometric operational taxonomic units 

分 类 号：R272[医药卫生—中医儿科学]