蒜芥茄染色体5 SrDNA、18 SrDNA和端粒序列位点检测及其核型分析  

Karyotype Analysis and Site Detection of 5 SrDNA,18 SrDNA and Telomere Sequence in the Chromosom of Solanum sisymbriifolium Lam.

作　　者：程捷 龚亚菊[1] 杜光辉[2] 蔚亚楠 黎志彬[1] 鲍锐[1] 桂敏[1] 吴丽艳[1] CHENG Jie;GONG Yaju;DU Guanghui;YU Ya’nan;LI Zhibin;BAO Rui;GUI Min;WU Liyan(Institute of Horticultural Crops,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Institute of Plant Resources,Yunnan University,Kunming 650500,China)

机构地区：[1]云南省农业科学院园艺作物研究所,昆明650205 [2]云南大学资源植物研究院,昆明650500

出　　处：《种子》2022年第7期16-20,26,共6页Seed

基　　金：国家自然科学基金地区基金项目(31960594);云南省应用基础研究面上项目(2019 FB 059);云南省农业基础研究联合专项面上项目(2018 FG 001-022);重大科技计划专项(202102 AE 090005)设施蔬菜标准化生产关键技术研究与应用。

摘　　要：本研究以蒜芥茄为试验材料,取其根尖进行染色体制片,基于端粒保守重复序列、5 SrDNA和18 SrDNA为探针进行荧光原位杂交,观察探针在染色体上标记的位置和信号的强弱,应用染色体核型分析软件进行图像采集和染色体长度的测量分析。结果表明,蒜芥茄的核型公式为2 n=2 x=24=24 m(2 SAT),染色体上各有一对5 SrDNA、18 SrDNA位点,端粒序列(TTTAGGG)位于染色体的两端。蒜芥茄为二倍体,染色体臂比值在1.05~1.48之间,为中部着丝粒染色体(m),最长染色体与最短染色体之比为1.42,核型不对称系数为54.73%,属于较原始的1 A型。In this study,S.sisymbriifolium Lam was chosen as test material,and its root tip was taken for chromosome section.Meanwhlie,the telomere conserved repeat sequence,5 SrDNA and 18 SrDNA were used as probes for fluorescence in situ hybridization(FISH)to observe the position of markers and signal intensity of probes on the chromosomes.Then,the software for chromosome karyotype analysis was used to collect image and measure the chromosome length.The results showed that the karyotype formula of S.sisymbriifolium Lam was 2 n=2 x=24=24 m(2 SAT).There were 1 pair of 5 SrDNA and 1 pair of 18 SrDNA loci on its chromosome,and telomere sequence(TTTAGGG)was located at both ends of the chromosome.S.sisymbriifolium Lam was a diploid with the chromosome arm ratio 1.05-1.48.Which it was a middle centromere chromosome(m).The ratio of the longest chromosome to the shortest chromosome was 1.42,and its karyotype asymmetry coefficient was 54.73%,belonging to the primitive type 1 A.

关 键 词：蒜芥茄 核型分析 端粒保守重复序列 5 SrDNA 18 SrDNA 

分 类 号：Q943.2[生物学—植物学] S641.1[农业科学—蔬菜学]