采用TMT技术解析辣椒雄性不育关键蛋白研究  

Analysis on Key Proteins of Pepper Male Sterility by TMT Technology

作　　者：覃成 杨仕梅 罗希榕 李唐燕 李靖 郭娅 陀海燕 涂韦波 邱化荣 QIN Cheng;YANG Shimei;LUO Xirong;LI Tangyan;LI Jing;GUO Ya;TUO Haiyan;TU Weibo;QIU Huarong(Engineering Research Center of Zunyi Pepper Germplasm Resources Conservation and Breeding Cultivation of Guizhou Province,Department of Modern Agriculture,Zunyi Vocational and Technical College,Zunyi Guizhou 563006,China;Key Lab of Zunyi Crop Gene Resource and Germplasm Innovation,Zunyi Academy of Agricultural Sciences,Zunyi Guizhou 563006,China)

机构地区：[1]遵义职业技术学院现代农业系/贵州省遵义辣椒种质资源保护及繁育种植工程研究中心,贵州遵义563006 [2]遵义市农业科学研究院/遵义市作物基因资源与种质创制重点实验室,贵州遵义563006

出　　处：《种子》2022年第7期42-50,共9页Seed

基　　金：贵州省科技支撑计划项目(黔科合支撑[2020]2258号、黔科合支撑[2021]一般267和黔科合支撑[2022]一般117);遵义市优秀青年科技创新人才培养项目(遵优青科[2018]6号);遵义市创新人才团队培养项目(遵市科人才[2019]4号);2020年度院级科研创新团队培养项目(遵职院办发[2020]87号)。

摘　　要：本研究通过对辣椒S 033-A保持系和S 033-B不育系的近等基因系辣椒进行蛋白质组学分析,揭示辣椒不育的关键蛋白。结果表明,保持系和不育系中蛋白总数为7667个,可定量蛋白为7629个,存在504个差异表达蛋白。保持系S 033-A有351个蛋白上调表达,不育系S 033-B有153个蛋白上调表达。结合生物信息学对差异表达蛋白进行亚细胞定位、保守结构域、GO和KEGG富集等进行分析,发现亚细胞定位主要在细胞质、细胞核和叶绿体,421条差异表达蛋白存在保守结构域,主要为谷胱甘肽S-转移酶、过氧化物酶、UDP-葡萄糖苷和UDP葡萄糖苷转移酶,GO富集在叶绿体、质体、类囊体中,KEGG富集主要参与光合作用、细胞色素P 450、单萜生物合成、类黄酮生物合成生化途径等。本研究从蛋白质组学角度发现了辣椒雄性不育相关的关键蛋白和代谢途径,揭示了辣椒雄性不育可能的分子机制,并鉴定了相关候选蛋白,为辣椒遗传育种奠定了理论基础。Proteomic analysis was conducted on pepper S 033-A maintainer line and S 033-B near-isogenic line to reveal the key proteins of pepper sterility.The results showed that the total number of identified proteins in maintainer line and sterile line was 7667,the quantified proteins were 7629,and there were 504 differentially expressed proteins.Out of these,351 proteins was upregulated expression in the maintainer line S 033-A,and 153 ones was upregulated expression in the sterile line S 033-B.Combined with bioinformatics,subcellular localization,conserved domain,GO and KEGG enrichment analysis of differentially expressed proteins were performed.It was found that subcellular localization was mainly in cytoplasm,nucleus and chloroplast,of which 421 proteins had conserved domains,which mainly were glutathione S-transferase,peroxidase,UDP-glucoside and UDP-glucoside transferase.GO enrichment was found within chloroplast,plastid and thylakoid.KEGG enrichment analysis showed that it was mainly involved in photosynthesis,cytochrome P 450,monoterpene biosynthesis and flavonoid biosynthesis pathways.In this study,we found the related key protein and metabolic pathways of pepper male sterility from the perspective of proteomics,and revealed the possible molecular mechanism of pepper male sterility lines and identified related candidate protein,which laid a theoretical foundation for pepper genetics and breeding.

关 键 词：辣椒 蛋白质组学 雄性不育 关键蛋白 

分 类 号：S641.3[农业科学—蔬菜学]