耐低温低氧水稻种质资源筛选  

Screening of Low Temperature and Hypoxic Resistance Germplasm Resources of Rice

作　　者：滕祥勇 王金明[1] 李鹏志[1] 林秀云[1] 孙强[1] TENG Xiangyong;WANG Jinming;LI Pengzhi;LIN Xiuyun;SUN Qiang(Rice Research Institute,Jilin Academy of Agricultural Science,Gongzhuling Jilin 136100,China)

机构地区：[1]吉林省农业科学院水稻研究所,吉林公主岭136100

出　　处：《种子》2022年第7期58-64,共7页Seed

基　　金：吉林省重点研发项目(20200402029 NC);国家重点研发计划(2017 YFD 0300608)。

摘　　要：本研究选用238份吉林省、黑龙江省水稻品种及自创高世代品系为供试材料,经室内简易程序(15℃恒温2 cm水深处理10 d)筛选,以萌发率、发芽率、发芽势为鉴定指标,初筛出59份耐低温低氧材料;对初筛出的材料进行室内精准鉴定,以12℃低温处理15 d后发芽率、发芽指数和活力指数为主鉴定指标,筛选出19份较耐冷材料,其中有8份材料表现强耐低温;以低氧胁迫下胚芽鞘长度为主鉴定指标,参考发芽率、苗高、缺氧反应指数,筛选出较耐低氧材料15份;低温低氧混合胁迫处理后有39份材料出苗成苗率较高;室内覆土试验显示,在催芽和干籽条件下成苗率均高于60%的有19份材料;田间自然鉴定,有21份材料表现出较高的出苗成苗率且出苗速度相对较快。综合混合胁迫、覆土试验和田间鉴定结果,筛选出16份较耐低温低氧材料:L-2、L-30、L-39、L-5、L-8、W-106、W-14、W-39、W-43、W-65、W-82、W-86、Z-32、Z-33、Z-59和Z-7。In this study, 238 rice varieties from Jilin Province and Heilongjiang Province and self-created high-generation strains were selected as the test materials, and were screened by a simple indoor program(15 ℃ constant temperature and 2 cm water depth for 10 days), and the germination rate, germination rate and germination potential were used as identification indicators. 59 low-temperature and hypoxia-resistant materials were initially screened;the initially screened materials were accurately identified indoors, and 19 relatively cold-resistant materials were screened out with germination rate, germination index and vigor index after low temperature treatment at 12 ℃ for 15 days as the main identification indicators, of which 8 materials showed strong low temperature resistance;15 materials were screened out with low temperature and hypoxia mixed with coleoptile length as the main identification index, germination rate, seedling height, and hypoxia response index were referenced;After low-temperature and hypoxia stress treatment, 39 materials had higher seedling emergence rate;indoor soil covering test showed that the seedling rate was higher than 60% under both germination and dry seed conditions;there were 21 materials by using natural identification in the field with higher seedling emergence rate and relatively fast emergence speed. Based on the results of mixed stress, soil covering test and field identification, 16 materials with low temperature and hypoxia resistance were screened out: L-2, L-30, L-39, L-5, L-8, W-106, W-14, W-39, W-43, W-65, W-82, W-86, Z-32, Z-33, Z-59 and Z-7.

关 键 词：低温 低氧 种质资源 筛选 

分 类 号：S511[农业科学—作物学]