天宫石斛快繁技术研究  

Study on Rapid Propagation Technology of Dendrobium aphyllum

作　　者：刘怡 王玥瑶 杨柳青 操赛雨 燕鑫 何碧珠[1,2] 郭梨锦 LIU Yi;WANG Yueyao;YANG Liuqing;CAO Saiyu;YAN Xin;HE Bizhu;GUO Lijin(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350000,China;Key Laboratory of Orchid Protection and Utilization,State Forestry and Grassland Administration,Fuzhou Fujian 350000,China;College of Resources and Environment,International Magnesium Institute,Fuzhou Fujian 350000,China)

机构地区：[1]福建农林大学园艺学院,福州350000 [2]兰科植物保护与利用国家林业与草原局重点实验室,福建福州350000 [3]资源与环境学院,国际镁营养研究所,福建福州350000

出　　处：《种子》2022年第7期138-143,F0003,共7页Seed

基　　金：福建省林业科技推广项目(2019 TG 17号)。

摘　　要：以天宫石斛荚果为外植体,探究天宫石斛种胚快繁技术。选择荚果外植体适合消毒时间,添加不同有机添加物诱导原球茎,调节生长调节剂种类及浓度配比,诱导增殖分化,添加适当浓度生根壮苗活性炭。结果表明,消毒10 min效果最佳,污染率为30%;添加土豆30 g/L培养基原球茎诱导效果最佳,诱导率为95%;6-BA 1.5 mg/L+NAA0.1 mg/L培养基增殖效果最佳,增殖系数为9.4;活性炭1.2 g/L生根壮苗效果最好,平均生根数达8.54。Using the pods of Dendrobium aphyllum as explants,the rapid propagation technology of Dendrobium aphyllum species was explored.Pod explants suitable for disinfection time was selected,adding different organic additives to induce protocorm,adjusting the type and concentration ratio of growth regulators,to induce proliferation and differentiation,and added an appropriate concentration of activated carbon for rooting and strong seedlings.The results showed that disinfection for 10 min had the best effect,and the contamination rate was 30%;adding potato 30 g/L medium had the best induction effect of protocorm,and when the induction rate was 95%;6-BA 0.5 mg/L+NAA 0.1 mg/L medium,the proliferation effect was the best,and the proliferation coefficient was 9.4;the effect of activated carbon 1.2 g/L was the best,and the average rooting number was 8.54.

关 键 词：天宫石斛 快繁 原球茎 增殖 生根 

分 类 号：S567.239[农业科学—中草药栽培]