赏食兼用百合京鹤高效组培再生体系的优化  

Optimization of High Efficient Tissue Culture Regeneration System for Ornamental and Edible Dual Purpose Lily-‘Jinghe’

作　　者：祁先宇 张文亮 杨盼盼[1] 徐雷锋[1] 明军[1] QI Xianyu;ZHANG Wenliang;YANG Panpan;XU Leifeng;MING Jun(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《中国蔬菜》2022年第8期57-62,共6页China Vegetables

基　　金：国家重点研发计划项目(2019YFD1001805,2018YFD1000405);中央级公益性科研院所基本科研业务费专项(IVF-BRF2020019);贵州省科技计划项目〔黔科合成果(2019)4235〕。

摘　　要：百合是重要的高档蔬菜和观赏花卉,基于鳞片的高效组织培养再生体系是百合种球规模化生产繁育的基础。本试验首次使用草甘膦为诱导分化剂,优化赏食兼用百合品种京鹤鳞片外植体的组培再生体系。结果表明:京鹤鳞片不定芽最佳诱导培养基为MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+2.5 mg·L^(-1)草甘膦+30 g·L^(-1)蔗糖,诱导系数为6.46,显著高于常规诱导培养;最佳继代增殖培养基为MS+0.5 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1)蔗糖,增殖系数为4.01;最佳鳞茎膨大培养基为MS+90 g·L^(-1)蔗糖;最佳生根培养基为1/2MS+0.3 mg·L^(-1) NAA+30 g·L^(-1)蔗糖,生根系数为23.59。Lily is an important high-grade vegetable and ornamental flower.It is based on scale high efficient tissue culture regeneration system which is the foundation for large-scale production and reproduction of lily bulbs.This experiment used glyphosate for the first time as differentiation-inducing agent to optimize tissue culture regeneration system taking scale as explant of lily variety‘Jinghe’with ornamental and edible dualpurpose.The result indicated that the optimal induction medium for‘Jinghe’scale adventitious bud was MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+2.5 mg·L^(-1) glyphosate+30 g·L^(-1) saccharose,and the induction coefficient was 6.46,which was significantly higher than that of the conventional induction medium.The optimal subculture medium was MS+0.5 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA+30 g·L^(-1) saccharose,and the proliferation coefficient was 4.01.The best medium for bulb expansion was MS+90 g·L^(-1) saccharose.The best rooting medium was 1/2MS+0.3 mg·L^(-1) NAA+30 g·L^(-1) saccharose.The rooting coefficient was 23.59.

关 键 词：百合 京鹤 组织培养 草甘膦 诱导分化 高效繁殖 

分 类 号：S644.1[农业科学—蔬菜学]