Lactoferrin-Conjugated Polylactic Acid Nanobubbles Encapsulated Perfluoropentane as a Contrast Agent for Ultrasound/Magnetic Resonance Dual-Modality Imaging  

作　　者：Liqiong Ding Pingsheng Li Liu He Fengnan Xu Jieqiong Ding Binhua Luo 

机构地区：[1]School of Pharmacy,Hubei University of Science and Technology,Xianning,437100,China [2]Pharmacy Department of the Second Affiliated Hospital,Hubei University of Science and Technology,Xianning,437100,China [3]School of Basic Medical Sciences,Hubei University of Science and Technology,Xianning,437100,China

出　　处：《Journal of Renewable Materials》2022年第3期767-780,共14页可再生材料杂志（英文）

基　　金：This work was financially supported by Initial Scientific Research Fund of Ph.D.in Hubei University of Science and Technology(BK202120).

摘　　要：The development of contrast agents that can be activated by multiple modes is of great significance for tumor diagnosis.In this study,the lactoferrin(Lf)-conjugated polylactic acid(PLLA)nanobubbles(Lf-PLLA NBs)were used to encapsulate liquid perfluoropentane(PFP)with the double emulsion method,creating PFP loaded(PFP/Lf-PLLA)NBs for the ultrasound/magnetic resonance dual-modality imaging of subcutaneous tumor.The parti-cle diameter and stability of nanobubbles were investigated by photon correlation spectroscopy.The biocompat-ibility of nanobubbles was preliminarily evaluated by cell proliferation and migration assay,hemolysis rate,and blood biochemistry analysis.A B-mode clinical ultrasound real-time imaging system was used to perform ultra-sonic imaging in vivo.Magnetic resonance imaging in vivo was applied with a clinical 3.0 T magnetic resonance imaging(MRI)scanner system.The mean particle diameter of PFP/Lf-PLLA NBs was 320.2±4.1 nm with a low polydispersity index(PDI,0.145±0.025),and the NBs were negatively charged(−11.4±0.4 mV).The transmis-sion electron microscopy(TEM)results showed that PFP/Lf-PLLA NBs exhibited highly monodispersed and pos-sessed an obvious spherical structure of nanocapsules.Nanobubbles had good stability at 4°C.Different concentrations of the PFP/Lf-PLLA NBs solution had no effect on the cell in cytotoxicity and cell migration,and the results of hemolysis rate and blood biochemistry assay also indicated the good biocompatibility of NBs.On the ultrasound/magnetic resonance imaging of tumor-bearing mice,PFP/Lf-PLLA NBs showed signifi-cantly enhanced contrast ability of tumor tissue.Therefore,PFP/Lf-PLLA NBs had great potential to be a contrast agent for tumor dual-modality imaging in vivo.

关 键 词：LACTOFERRIN polylactic acid NANOBUBBLES contrast agent ultrasound/magnetic resonance imaging 

分 类 号：R445.2[医药卫生—影像医学与核医学]