荧光定量PCR检测法在阴道加德纳菌检测中的应用价值  

Application value of fluorescent quantitative PCR in detection of Gardnerella vaginalis

作　　者：林爽 LIN Shuang(Department of Clinical Laboratory,Chifeng Hospital,Chifeng 024000,China)

机构地区：[1]赤峰市医院检验科,024000

出　　处：《中国实用医药》2022年第17期87-89,共3页China Practical Medicine

摘　　要：目的 分析荧光定量聚合酶链反应(PCR)检测法对阴道内加德纳菌的诊断效果。方法 选择30例细菌性阴道炎患者作为实验组,另选择30例体检的健康女性作为对照组。两组均采用实时荧光定量PCR检测法检测阴道加德纳菌,对比两组检测结果。结果 实验组标本进行实时定性PCR检测, PCR扩增产物经过电泳检测后发现111 bp特异性条带。对其进行序列分析,实验结果与同源性对比发现,扩增DNA片段与序列符合率100%。依据Amsel诊断标准,实验组30份样本中加德纳菌阳性标本数为28份,阳性率为93.33%;对照组30份样本中加德纳菌阳性标本数为12份,阳性率为40.00%。实验组加德纳菌阳性率高于对照组,差异有统计学意义(P<0.05)。结论 在细菌性阴道炎检测中应用荧光定量PCR检测法检测加德纳菌效果明确,可以为医生提供诊断依据,具有临床应用价值。Objective To analyze the effect of fluorescent quantitative polymerase chain reaction(PCR)in diagnosis of Gardnerella vaginalis.Methods 30 patients with bacterial vaginosis were selected as the experimental group,and another 30 healthy women who underwent physical examinations were selected as the control group.Both groups were detected by real-time fluorescent quantitative PCR method to detect Gardnerella vaginalis,and the detection results of the two groups were compared.Results After the specimens of the experimental group were subjected to real-time qualitative PCR,the PCR amplification products could be found 111 bp specific bands after electrophoretic detection.Sequence analysis was performed,and the experimental results were compared with homology,and the amplified DNA fragments were found to have 100%sequence conformity.According to the Amsel diagnostic criteria,the number of Gardnerella positive specimens was 28 out of 30 samples in the experimental group,with a positive rate of 93.33%;in the control group,the number of Gardnerella positive specimens was 12 out of 30 samples,with a positive rate of 40.00%.The positive rate of Gardnerella of the experimental group was higher than that of the control group,and the difference was statistically significant(P<0.05).Conclusion The detection of Gardnerella vaginalis by fluorescent quantitative PCR is effective in the detection of bacterial vaginitis.It can provide diagnostic basis for doctors and has clinical application value.

关 键 词：实时荧光定量 聚合酶链反应检测 加德纳菌 细菌性阴道病 

分 类 号：R446.5[医药卫生—诊断学]