罗氏沼虾转录组SSR标记筛选及其与体质量相关性分析  

SCREENING OF TRANSCRIPTOME SSR MARKER OF MACROBRACHIUM ROSENBERGII AND ANALYSIS OF ITS CORRELATION WITH BODY WEIGHT

作　　者：陈雪峰[1,2] 王春琳 李荣华[2] 顾志敏[1] 徐宾朋[1] 程海华 彭菲 CHEN Xue-Feng;WANG Chun-Lin;LI Rong-Hua;GU Zhi-Min;XU Bin-Peng;CHENG Hai-Hua;PENG Fei(National Genetic Breeding Center for Macrobrachium rosenbergii,Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China;School of Marine Science,Ningbo University,Ningbo 315211,China)

机构地区：[1]浙江省淡水水产研究所,国家罗氏沼虾遗传育种中心,湖州313001 [2]宁波大学海洋学院,宁波315211

出　　处：《水生生物学报》2022年第8期1150-1159,共10页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2018YFD0901303)资助。

摘　　要：研究通过转录组测序对罗氏沼虾(Macrobrachium rosenbergii)SSR标记进行了发掘,获得SSR位点18592个。随机选取了63个SSR位点设计引物并利用10个个体进行多态性位点筛选,共获得31个具有多态的SSR标记,利用其中19个SSR标记对全同胞家系群(FS)119尾虾及孟加拉F3代野生群(MJL)199尾虾的群体遗传多样性进行分析,并对SSR位点与体质量性状进行关联性分析。结果表明:FS群体共检测到40个等位基因,平均等位基因数为2.1053,平均观测杂合度0.4525,平均期望杂合度0.3804,平均多态信息含量0.3076,处于中度遗传多态水平;MJL群体共检测到65个等位基因,平均等位基因数为3.4211,平均观测杂合度0.4105,平均期望杂合度0.4496,平均多态信息含量0.3882,处于中度遗传多态水平。19个SSR位点与FS群体体质量没有相关性(P>0.05),而在MJL群体中, 4个SSR位点与体质量显著相关(P<0.05)。对差异显著的位点进行不同基因型体质量性状的多重比较, MR28位点277/285基因型体质量均值极显著高于277/289和285/285基因型(P<0.01),显著高于285/289、273/289、270/273和285/293基因型(P<0.05);MR32位点266/266和266/270基因型体质量均值显著高于270/270基因型(P<0.05);MR34位点210/214基因型体质量均值显著高于210/210和214/214基因型(P<0.05);MR45位点174/190基因型体质量均值显著高于182/182和182/190基因型(P<0.05)。研究结果为罗氏沼虾分子标记辅助育种奠定了基础。In this study,the SSR markers of Macrobrachium rosenbergii were excavated by transcriptome sequencing,and 18592 SSR loci were obtained.63 SSR loci were selected and 10 individuals were utilized to screen the polymorphic loci,and 31 SSR markers with polymorphism were obtained finally.Among which,19 SSR markers were used to analyze the population genetic structure of 119 shrimps from the full-sib family group(FS)and 199 shrimps from the F3 wild group of Bangladesh(MJL),and the correlation between SSR loci and body mass traits was analyzed.The results showed that 40 alleles were detected in FS population,with an average alleles number of 2.1053,an average observed heterozygosity of 0.4525,an average expected heterozygosity of 0.3804,an average polymorphism information content of 0.3076,which was moderate genetic polymorphism;A total of 65 alleles were detected in MJL population,with an average alleles number of 3.4211,an average observed heterozygosity of 0.4105,an average expected heterozygosity of 0.4496,and an average polymorphism information content of 0.3882,which was also moderate genetic polymorphism.There was no correlation between 19 SSR loci and body weight in FS population(P>0.05),but 4 SSR loci were significantly correlated with body weight in MJL population(P<0.05).For the loci with significant differences,body weight traits of different genotypes were analyzed by multiple comparison,which showed that the mean body weight of MR28277/285 genotype was significantly higher than that of 277/289 and 285/285 genotypes(P<0.01),and significantly higher than that of 285/289,273/289,270/273 and 285/293 genotypes(P<0.05);The mean body mass of genotypes 266/266 and 266/270 at MR32 was significantly higher than that of genotypes 270/270(P<0.05).The mean body mass of genotypes of 210/214 at MR34 was significantly higher than that of genotypes 210/210 and 214/214(P<0.05).The mean body mass of genotypes of 174/190 at MR45 was significantly higher than that of genotypes 182/182 and 182/190(P<0.05).The results lay a foun

关 键 词：转录组测序 SSR标记 关联分析 体质量 罗氏沼虾 

分 类 号：Q523.8[生物学—生物化学] S917[农业科学—水产科学]