按不同时间方案注射的乙肝免疫球蛋白与乙肝疫苗对小鼠脾脏淋巴细胞亚群的影响  

Effect of hepatitis B immunoglobulin and hepatitis B vaccine injected at different time intervals on spleen lymphocyte subsets in mice

作　　者：闫柳柳 王爽[1] 张晓[2] 魏涛[3] 韩迪(指导)[4] 张磊(指导)[2] YAN Liuliu;WANG Shuang;ZHANG Xiao;WEI Tao;HAN Di;ZHANG Lei(Qingdao University,Qingdao 266000,China)

机构地区：[1]青岛大学,青岛266000 [2]青岛大学附属青岛市市立医院,青岛266000 [3]青岛大学附属青岛市妇女儿童医院,青岛266000 [4]青岛大学附属医院,青岛266000

出　　处：《中国免疫学杂志》2022年第11期1294-1299,1305,共7页Chinese Journal of Immunology

基　　金：国家自然科学基金青年基金项目(81502900)。

摘　　要：目的:观察按不同时间方案注射的乙肝免疫球蛋白(HBIG)与乙肝疫苗对小鼠脾脏淋巴细胞亚群的影响。方法:饲养BALB/c小鼠120只(雌雄各60只),将小鼠按HBIG与第1针乙肝疫苗接种时间间隔分为三组(0周组、2周组、4周组),每组小鼠按接种的乙肝疫苗剂量分为2个亚组(1μg组、2μg组)。每只小鼠于第0周注射50 IU HBIG。所有小鼠于第3针乙肝疫苗接种后4周分离脾脏淋巴细胞,使用流式细胞仪检测淋巴细胞亚群(CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、CD3−CD19^(+))比例。结果:0、2、4周组比较,两亚组(1μg、2μg)显示,与体液免疫相关的CD3−CD19^(+)B淋巴细胞所占比例在雄、雌鼠4周组中均分别高于0、2周组,差异有统计学意义;与细胞免疫相关的T淋巴细胞及其亚群(CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+))均未显示4周组高于其他两组。乙肝疫苗1μg组、2μg组比较,在雄、雌鼠中均显示4周组中2μg组CD3−CD19^(+)B淋巴细胞所占比例高于1μg组(P<0.05)。与细胞免疫相关的T淋巴细胞亚群未显示出2μg组高于1μg组的趋势。结论:小鼠注射HBIG的前提下,推迟接种乙肝疫苗及增加乙肝疫苗剂量有利于B淋巴细胞的增殖,推迟接种乙肝疫苗对T淋巴细胞亚群比例无明显影响。Objective:To observe the effect of hepatitis B immunoglobulin(HBIG)and hepatitis B vaccine injected at different time intervals on proliferation of splenic lymphocyte subsets in mice.Methods:120 BALB/c mice(60 males and 60 females)were fed.According to the time interval between HBIG and the first dose of hepatitis B vaccine,mice were divided into three groups(0-week group,2-week group and 4-week group).Mice in each group were divided into two subgroups(1μg group and 2μg group)according to the dose of hepatitis B vaccine administrated.Each mouse was injected with 50 IU HBIG at 0 week.Spleen lymphocytes were isolated from all mice 4 weeks after the third dose of hepatitis B vaccine.Proportion of lymphocyte subsets(CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+),CD3−CD19^(+))were detected by flow cytometry.Results:Comparation of 0,2,4 week groups,proportion of CD3−CD19^(+)B lymphocytes related to humoral immunity in the two subgroups(1μg group and 2μg group)were higher in 4-week group than that in 0-and 2-week group,and the differences were statistically significant.Proportion of T lymphocytes and its subsets(CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+))related to cellular immunity were not higher in 4-week group than in the other two groups.Compared 1μg group with 2μg group,proportion of CD3−CD19^(+)B lymphocytes in 2μg group was higher than that in 1μg group in both male and female mice in 4-week group(P<0.05).Proportion of T lymphocyte subsets related to cellular immunity did not show that it was higher in 2μg group than in 1μg group.Conclusion:After injection of HBIG to mice,delayed administration of hepatitis B vaccine and increasing dose of hepatitis B vaccine contribute to proliferation of B lymphocytes,while delayed administration of hepatitis B vaccine has no obvious effect on proportion of T lymphocyte subsets.

关 键 词：乙肝免疫球蛋白 乙肝疫苗 淋巴细胞 脾脏 BALB/C小鼠 

分 类 号：R392.1[医药卫生—免疫学]