犊牛溶血曼氏杆菌疫苗免疫安全性及效果评价  

Safety Evaluation and Immune Effect Monitoring of Mannheimia haemolytica Vaccine in Calves

作　　者：孟文文 商云鹏 赵卓[2] 高荣荣 曹杰[1] MENG Wen-wen;SHANG Yun-peng;ZHAO Zhuo;GAO Rong-rong;CAO Jie(College of Veterinary Medicine,China Agricultural University,Beijing 100193;Beijing Centre Biology Co.Ltd,Beijing 102600)

机构地区：[1]中国农业大学动物医学院,北京100193 [2]北京生泰尔科技股份有限公司,北京102600

出　　处：《中国奶牛》2022年第8期24-28,共5页China Dairy Cattle

基　　金：宁夏重点研发计划项目“宁夏奶牛养殖区域重大疾病综合防控技术集成与创新”(21BEF02019)。

摘　　要：选取2~6月龄健康荷斯坦犊牛2500头,免疫牛溶血曼氏杆菌灭活疫苗,监测免疫后不良反应。结果表明,犊牛免疫后3d内采食量下降12%,随后恢复;免疫后5d内,约有65%的牛出现体温一过性升高,95%的犊牛出现注射部位皮下肿胀,免疫后2周内自行消退。2月龄犊牛二免后14d抗体水平最高,合格率及阳性率达100%,随后开始下降,免疫后6个月抗体滴度低于临界值。免疫后6个月内犊牛肺炎发病及死亡牛只溶血曼氏杆菌未检出。溶血曼氏杆菌灭活疫苗A1型M164株临床应用安全,2月龄犊牛二免后5个月进行加强免疫。Two thousand five hundred healthy Holstein calves aged 2~6 months were selected to be immunized with the inactivated Mannheimia haemolytica vaccine to monitor the adverse reactions after immunization.The results showed that the feed intake of calves decreased by 12%within 3 days after immunization,and then recovered;within 5 days after immunization,about 65%of calves experienced a transient increase in body temperature,and95%of calves showed subcutaneous swelling at the injection site.The swelling subsided spontaneously within 2 weeks after immunization without other abnormalities.The antibody level of 2-month-old calves were the highest14 days after the second immunization,with a pass rate and a positive rate of 100%,and then began to decline.The antibody titer was lower than the critical value at 6 months after immunization.Within 6 months after immunization,the incidence of pneumonia and death in calves were not detected in Mannheimia haemolytica.The inactivated Mannheimia haemolytica vaccine A1 M164 strain is safe for clinical application,and booster immunization is given to two-month-old calves 5 months after the second immunization.

关 键 词：溶血曼氏杆菌 犊牛 安全性 免疫 抗体 

分 类 号：S858.23[农业科学—临床兽医学]