佤药益气通络方单药和组方成分对比及急性毒性研究  

Comparison of Components of Wa Nationality Medicine Yiqi Tongluo Formula and Every Herb of It and Inspect the Acute Toxicity

作　　者：海青山 赵杰 左爱学 金亚菊 李明明 高磊 李仪杰 王丽欢 温伟波 HAI Qingshan;ZHAO Jie;ZUO Aixue;JIN Yaju;LI Mingming;GAO Lei;LI Yijie;WANG Lihuan;WEN Weibo(Department of Basic Medical Sciences,Yunnan University of Chinese Medcine,Kunming 650500,China;First Department of Clinical Medical,Yunnan University of Chinese Medcine,Kunming 650500,China;J.Department of Chinese Medcine,Yunnan University of Chinese Medcine,Kunming 650500,China;Department of Acupuncture and Massage and Rehabilitation,Yunnan University of Chinese Medcine,Kunming 650500,China;Department of Ethnomedicine,Yunnan University of Chinese Medcine,Kunming 650500,China)

机构地区：[1]云南中医药大学基础医学院,云南昆明650500 [2]云南中医药大学第一临床医学院,云南昆明650500 [3]云南中医药大学中药学院,云南昆明650500 [4]云南中医药大学针灸推拿康复学院,云南昆明650500 [5]云南中医药大学民族医药学院,云南昆明650500

出　　处：《云南中医学院学报》2022年第1期59-64,共6页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省科技厅科技计划项目(2019ZF005);云南省科学技术厅-云南中医药大学应用基础研究联合专项资金项目(202001AZ070001-026)。

摘　　要：目的对益气通络方煎煮过程中是否有新的化合物产生进行分析,并对全方的急性毒性进行研究。方法通过高效液相色谱法(HPLC),将益气通络方混提的样品和单独煎煮的地桃花、鸡根、滇鸡血藤混合后的溶液进行对比,分析成分差异;通过LD_(50)和最大给药量实验检查益气通络方的急性毒性。结果1.分别提取地桃花、鸡根、滇鸡血藤3味药单独进行HPLC检测,流速1.0 m L/min,柱温30℃,分别以210 nm、254 nm、280 nm和320nm波长进行检测,254 nm的吸收图谱最理想;2.将地桃花、鸡根、滇鸡血藤3味药水提液按比例混匀,在254 nm波长下与整方混提的HPLC吸收图谱进行比较,2个谱图的吸收峰紫外光谱对应的位置和形态基本一致,除少数吸收峰的幅度略有差异外,波形的拟合度较高;3.检测益气通络方LD_(50),8个浓度梯度均未见动物死亡和明显的毒性反应;4.益气通络方溶解至最大浓度,一次性给予最大灌胃量,折合给药量为246.9 g/kg,仍未见到动物死亡和明显毒性反应。结论1.益气通络方和地桃花、鸡根、滇鸡血藤单药煎煮后混合相比,并未出现明显的成分改变;2.在急性毒性动物实验中,益气通络方未见明显的不良反应。Objective To analyze whether there are new compounds in the decoction of Yiqi Tongluo formula,and study the acute toxicity of the whole formula.Methods Through high performance liquid chromatography(HPLC),compare the samples of Yiqi Tongluo formula with the mixed solution of separately decocted Urena lobata,Polygala arillata and Caulis Kadsurae,and analyze the differences of components;Study the acute toxicity of Yiqi Tongluo formula by examining LD_(50) and maximum dose experiment.Results 1.The three samples of Urena lobata,Polygala arillata and Caulis Kadsurae were tested by HPLC separately,the flow velocity was 1.0 m L/min,the column temperature was 30℃,and the wavelengths of 210 nm,254 nm,280 nm and 320 nm were detected respectively,the best absorption spectrum was 254 nm.2.Mix the solutions of Urena lobata,Polygala arillata and Caulis Kadsurae in proportion,and compared with Yiqi Tongluo formula by HPLC in the wavelength of 254 nm.The positions and forms of the absorption peaks of the UV spectra in the two spectrogram were basically the same,except that the amplitudes of minority absorption peaks were slightly different,the fitting degree of the waveform was high.3.No animal death and none obvious toxic reaction were found in the 8 concentration gradients of Yiqi Tongluo formula when determine LD_(50).4.Yiqi Tongluo formula was dissolved to the maximum concentration,and the maximum intragastric dose was given at one time,as high as to 246.9 g/kg,also no animal death and obvious toxic reactions were seen.Conclusion:1.Compared with the mixture of the Urena lobata,Polygala arillata and Caulis Kadsurae which were decocted separately,Yiqi Tongluo recipe had no significant changes in composition.2.In the animal experiment of acute toxicity,Yiqi Tongluo formula has no obvious adverse reactions.

关 键 词：佤药 益气通络方 地桃花 鸡根 滇鸡血藤 急性毒性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]