安五脂素对D-gal致衰老小鼠胸腺的保护作用及其相关机制  

Anwulignan protects thymus of aging mice induced by D-galactose and its related mechanism

作　　者：李欣 孙靖辉[1] 王春梅[1] 陈建光[1] 敬舒[2] 李贺(指导)[1] LI Xin;SUN Jinghui;WANG Chunmei;CHEN Jianguang;JING Shu;LI He(College of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院,吉林132013 [2]北华大学附属医院,吉林132011

出　　处：《中国免疫学杂志》2022年第12期1451-1457,共7页Chinese Journal of Immunology

基　　金：吉林省科技发展计划项目(20200404053YY,20200404022YY);吉林省科技创新发展计划项目(20200502084);2020年度吉林省中医药科技项目(2020121);2020年省预算内基本建设资金项目(2020C033-2)。

摘　　要：目的:观察安五脂素对D-半乳糖(D-gal)致衰老小鼠胸腺的保护作用并探讨其相关机制。方法:将ICR小鼠随机分为5组,空白对照组(CON,灌胃羧甲基纤维素钠溶液,皮下注射生理盐水),D-gal模型组(MOD,灌胃羧甲基纤维素钠溶液,皮下注射220 mg/kg D-gal),安五脂素低、中、高剂量组(分别灌胃安五脂素1、2和4 mg/kg,皮下注射220 mg/kg D-gal),1次/d,10 ml/kg,连续造模及给药42 d。计算胸腺指数,观察胸腺组织病理变化,测定血清中IL-2和IFN-γ含量,胸腺组织中SOD活力和MDA含量,应用Western blot法检测胸腺组织中Keap1、Nrf2、HO-1、Caspaes3、Bcl2和Bax等蛋白的表达情况。结果:安五脂素能显著提高胸腺指数并改善受损胸腺结构,提高IL-2和IFN-γ含量,提高胸腺组织中SOD活力,降低MDA含量,上调胸腺组织中Nrf2,HO-1和Bcl2的表达,降低Keap1、Caspase3和Bax的表达水平。结论:安五脂素对D-gal所致的小鼠胸腺损伤具有保护作用,其机制可能与其抗氧化和抗凋亡作用有关。Objective:To observe the protective effect of Anwulignan on the thymus of aging mice induced by D-galactose(D-gal)and explore its related mechanism.Methods:ICR mice were randomly divided into five groups,blank control group(CON,gavage of sodium carboxymethyl cellulose solution,subcutaneous injection of normal saline),D-gal model group(MOD,gavage of sodium carboxymethyl cellulose solution,subcutaneous injection of 220 mg/kg D-gal),and low,medium and high dose group(gavage of Anwulignan 1,2 and 4 mg/kg,respectively,subcutaneous injection of 220 mg/kg D-gal),once a day,10 ml/kg,for 42 days.Thymus index was calculated,pathological changes of thymus tissue were observed,the serum IL-2 and IFN-γcontents,the thymus SOD activity and MDA content were determined,and the expressions of the thymus Keap1,Nrf2,HO-1,Caspaes3,Bcl2 and Bax proteins were detected by Western blot.Results:Anwulignan significantly increased the thymus index and improved its damaged structure,increased IL-2 and IFN-γcontents,increased the thymus SOD activity,reduced MDA content,up-regulated the expressions of the thymus Nrf2,HO-1 and Bcl2,and reduced the expressions of the thymus Keap1,Caspase3 and Bax.Conclusion:Anwulignan has protective effect on thymus injuries induced by D-gal,and its mechanism may be related to its antioxidant and anti-apoptotic effects.

关 键 词：安五脂素 氧化 凋亡 

分 类 号：R285.5[医药卫生—中药学]