一次性尿多巴胺生物医学传感器研发  

Research and development of disposable urinary dopamine biomedical sensor

作　　者：朱春楠[1,2,3] 陈道明 刘晓军 刘超[1,2,3] 张朱珊莹 郑冬云 ZHU Chunnan;CHEN Daoming;LIU Xiaojun;LIU Chao;ZHANG Zhushanying;ZHENG Dongyun(College of Biomedical Engineering,SouthCentral Minzu University,Wuhan 430074,China;Hubei Key Laboratory of Medical Information Analysis&Tumor Diagnosis and Treatment,Wuhan 430074,China;Key Laboratory of Brain Cognitive Science(SouthCentral Minzu University),State Ethnic Affairs Commission,Wuhan 430074,China)

机构地区：[1]中南民族大学生物医学工程学院,湖北武汉430074 [2]医学信息分析及肿瘤诊疗湖北省重点实验室,湖北武汉430074 [3]脑认知科学实验室国家民委重点实验室(中南民族大学),湖北武汉430074

出　　处：《传感器与微系统》2022年第8期76-79,84,共5页Transducer and Microsystem Technologies

基　　金：国家自然科学基金资助项目(22004134,21804146);湖北省自然科学基金资助项目(2020CFB313);中央高校基本科研业务费专项资金资助项目(CZQ21025,CPT22022,CZY20006)。

摘　　要：使用一次性枪头构造空腔,通过向其中填充石墨粉及石蜡油混合物,制备出石墨碳糊微电极(GCPME),并借助于简单可控的吸附法,将阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)修饰到GCPME表面,制得CTAB/GCPME。电化学表征结果表明:CTAB/GCPME对多巴胺(DA)的电化学氧化具有显著的催化作用。在最佳检测条件下,DA在CTAB/GCPME上的氧化峰电流与其浓度在2.0×10^(-7)-1.0×10^(-4)mol/L范围内呈良好线性关系,检出限低至4.0×10^(-8)mol/L(S/N=3)。此外,CTAB/GCPME用于DA检测还具有良好的特异性、稳定性和重现性,且可用于人体尿样中DA含量的准确快速检测。CTAB/GCPME成本低、制备简单、性能好,可用作一次性尿DA生物医学传感器件,具有良好的生物医学应用前景。A disposable pipette gun head is used to construct a cavity,which is filled with a mixture of graphite powder and paraffin oil to prepare a graphite carbon paste microelectrode(GCPME).Through a simple and controllable adsorption method,cetyl trimethyl ammonium bromide(CTAB)that is a cationic surfactant is modified on the surface of GCPME and the CTAB/GCPME is fabricated.The electrochemical characterization results show that CTAB/GCPME has an obvious catalytic effect on electrochemical oxidation of dopamine(DA).Under the optimal detection conditions,the oxidation peak current of DA on CTAB/GCPME and its concentration has a good linear relationship in the concentration range of 2.0×10^(-7)~1.0×10^(-4)mol/L with a low detection limit of 4.0×10^(-8)mol/L(S/N=3).In addition,CTAB/GCPME has good specificity,stability and reproducibility for DA detecting,and can be used for accurate and rapid detection of DA in human urine samples.CTAB/GCPME has the advantages of low cost,simple preparation and good performance.It can be used as a disposable urinary DA biomedical sensor and has a good biomedical application prospect.

关 键 词：石墨碳糊微电极 十六烷基三甲基溴化铵 多巴胺检测 生物医学传感器 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学]