An excimer ‘ON–OFF’ switch based on telomeric G-quadruplex and rGO for trace thrombin detection  

作　　者：Long Zhao Farid Ahmed Hai Xiong 

机构地区：[1]Institute for Advanced Study,Shenzhen University,Shenzhen 518060,China [2]College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,China

出　　处：《Chinese Chemical Letters》2022年第9期4243-4247,共5页中国化学快报（英文版）

基　　金：supported by the Science and Technology Innovation Commission of Shenzhen,China(Nos.KQJSCX20180328095517269 and JCYJ20210324095607021)。

摘　　要：In the recent decade,GO has emerged as an amazing 2D nanomaterial for developing DNA-based biosensors due to its fluorescence quenching properties,whereas similar research based on rGO was reported rarely.Herein,a novel multi-pyrene functionalized G-rich DNA probe based on the screened rGO showed much higher fluorescence quenching efficiency and excimer emission than that of universal GO.Different from the universal thrombin detection of the G4-forming aptamer-TBA(GGTTGGTGTGGTTGG),the original telomeric sequence is used in this study.The excimer emission“ON-OFF”switch amplified the response of thrombin detection is as low as 50 units.Furthermore,for four pyrene moieties that are sited in a crowded steric circumstance,the melting temperature(T_(m))values and molecular dynamics simulations showed a positive effect on duplex G-quadruplex or _(G)DNA·_(c)DNA stability,without disturbing its helix structure.

关 键 词：G-quadruplexr GOMulti-pyrene excimer BIOSENSOR Thrombin detection 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] R313[自动化与计算机技术—控制科学与工程]