黄连素对脑出血大鼠脑组织免疫炎症反应的影响  

Effect of berberine on immune inflammatory response after intracerebral hemorrhage in SD rats

作　　者：任怡君 陈玺倩 卢伟[1] REN Yijun;CHEN Xiqian;LU Wei(Department of Neurology,The Second Xiangya Hospital,Central South University,Changsha,Hunan 400012,China)

机构地区：[1]中南大学湘雅二医院神经病学科,湖南长沙400012

出　　处：《湖南中医药大学学报》2022年第8期1261-1265,共5页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金项目(S2020JJMSXM3101)。

摘　　要：目的探究黄连素对脑出血(intracerebral hemorrhage,ICH)大鼠脑组织中免疫炎症反应的影响。方法48只SD大鼠随机分为假手术(Sham)组、模型(ICH)组、黄连素(BBR)组,每组分为24 h及72 h两个时间点,每个亚组各8只。采用Ⅳ型胶原酶法构建ICH大鼠模型,BBR组给予黄连素200 mg/(kg·d)灌胃。对各组大鼠进行改良Garcia评分,Western blot法检测基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-6(interleukin-6,IL-6)蛋白表达水平,免疫荧光染色法检测小胶质细胞活化和极化的情况。结果与相同时间点的Sham组相比,ICH组改良Garcia评分明显升高(P<0.05),脑组织中出现明显血肿,脑组织中MMP-9、TNF-α和IL-6表达明显升高(P<0.05),脑组织中小胶质细胞活化。与各时间点ICH组相比,BBR组改良Garcia评分呈下降趋势,但差异无统计学意义(P>0.05)。24 h时,与ICH组相比,BBR组脑组织中MMP-9表达明显降低(P<0.05),TNF-α和IL-6表达降低,但差异无统计学意义(P>0.05),M2型小胶质细胞比例升高;72 h时,与ICH组相比,BBR组脑组织中MMP-9和TNF-α表达明显降低(P<0.05),IL-6表达降低,但差异无统计学意义(P>0.05),M2型小胶质细胞比例升高。结论黄连素可通过影响中枢神经系统免疫反应,下调炎症因子表达、促进小胶质细胞极化为M2抗炎亚型,发挥神经保护作用。Objective To investigate the influence of berberine on immune and inflammation responses in brain tissues of rats with intracerebral hemorrhage(ICH).Methods A total of 48 SD rats were randomly divided into sham-operated group(Sham group),ICH model group(ICH group),and berberine(BBR)group.Each group was divided into 24 h and 72 h time points.The IV collagenase was used to construct experimental ICH models in rats.The BBR group was given berberine 200 mg/(kg·d)by gavage,once a day.Modified Garcia score was performed on rats in each group.Western blot method was used to detect the protein expression levels of matrix metalloproteinase-9(MMP-9),tumor necrosis factorα(TNF-α)and interleukin-6(IL-6).Fluorescence immunostaining was used to detect the activation and polarization of microglia.Results Compared with the Sham group at each time point,the modified Garcia scores were significantly increased(P<0.05)in the ICH group.Obvious hematoma appeared in the brain tissue,and the expression levels of MMP-9,IL-6 and TNF-αin the brain tissue were increased significantly(P<0.05).Microglia were activated after ICH.Compared with the ICH model group at each time point,the modified Garcia scores in the BBR group showed a downward trend,which was not of statistically significance(P>0.05).At 24 h,compared with the ICH group,the expression of MMP-9 in the BBR group was significantly decreased(P<0.05),and the expression levels of TNF-αand IL-6 were decreased,which was not of statistically significance(P>0.05).The proportion of M2-type microglia increased.At 72 h,compared with the ICH group,the expression levels of MMP-9 and TNF-αin the BBR group were significantly decreased(P<0.05),and the expression of IL-6 decreased,which was not of statistically significance(P>0.05).The proportion of M2-type microglia increased.Conclusion Berberine exhibits neuroprotective effects by influencing the immune response of the central nervous system through down-regulating the expression of inflammatory factors and promoting the polarization of mic

关 键 词：脑出血 免疫炎症反应 小胶质细胞 黄连素 神经保护 炎症因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]