粉美人萱草的快繁技术和大田种植  

Rapid Propagation Technology and Field Production of Hemerocallis fulva cv.’Fenmeiren’

作　　者：吕秀立 于泽群 陈香波 傅仁杰 缪珊珊 杜安 Xiuli Lü;Zequn Yu;Xiangbo Chen;Renjie Fu;Shanshan Miao;An Du(Shanghai Academy of Landscape Architecture Science and Planning,Shanghai 200232,China;Shanghai Gardening-Landscaping Construction Co,Ltd,Shanghai 200333,China;Shanghai Landscape Architecture Design Research Institute Co,Ltd,Shanghai 200031,China)

机构地区：[1]上海市园林科学规划研究院,上海200232 [2]上海园林绿化建设有限公司,上海200333 [3]上海市园林设计研究总院有限公司,上海200031

出　　处：《植物学报》2022年第3期350-357,共8页Chinese Bulletin of Botany

基　　金：国家林业和草原局林草国家创新联盟自筹研发项目(No.GLM[2021]101)。

摘　　要：以粉美人萱草(Hemerocallis fulva cv.‘Fenmeiren’)的花茎为外植体进行离体培养,该研究成功建立了粉美人萱草组培快繁技术。结果表明,6月获得的外植体用浓度为15%(v/v)的次氯酸钠溶液消毒8分钟,外植体存活率达95%;最佳增殖培养基为MS+1.0 mg·L^(-1)6-BA+0.004 mg·L^(-1)TDZ+0.1 mg·L^(-1)NAA,培养30天后,月增殖系数达2.9;壮苗培养基为MS+0.1 mg·L^(-1)6-BA+0.1 mg·L^(-1)IBA,在该培养基中,组培苗不再分化,长势健壮;最佳生根培养基为1/2MS+0.4 mg·L^(-1)IBA+20 g·L^(-1)蔗糖,生根率达95%;移栽基质采用珍珠岩:草炭=1:2(v/v),通过精细化管理,成活率可达85%,出圃合格率为75%。目前已实现规模化繁殖,并生产组培苗2.0×10^(5)株,大田种植表现良好。Using the scape as explants,we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv.‘Fenmeiren’.Our results showed that the survival rate of explant was 95%,which explants were obtained in June and sterilized in the 15%(v/v)sodium hypochlorite solution for 8 minutes.The best proliferation medium was MS+1.0 mg·L^(-1)6-BA+0.004 mg·L^(-1) TDZ+0.1 mg·L^(-1) NAA.After 30 days of cultivation,the monthly proliferation coefficient reached 2.9.On the medium of MS+0.1 mg·L^(-1)6-BA+0.1 mg·L^(-1) IBA,the tissue culture seedlings did not differentiate and grew vigorously.The optimal rooting medium was 1/2MS+0.4 mg·L^(-1) IBA+20 g·L^(-1) sucrose,and the rooting rate was 95%.by use of perlite:peat=1:2(v/v)as the transplanting matrix,the survival rate of transplanting was 85% and the qualified rate was 75%.At present,the large-scale production of this cultivar has been achieved and 2.0×10^(5) tissue culture seedlings were produced,which all performed well in the field.

关 键 词：粉美人萱草 快繁技术 规模化繁殖 大田种植 

分 类 号：S682.19[农业科学—观赏园艺]