Inherently hydrophilic mesoporous channel coupled with metal oxide for fishing endogenous salivary glycopeptides and phosphopeptides  

作　　者：Zixing Xu Yonglei Wu Xufang Hu Chunhui Deng Nianrong Sun 

机构地区：[1]Department of Chemistry,Fudan University,Shanghai 200433,China [2]Department of Gastroenterology and Hepatology,Zhongshan Hospital,Fudan University,Shanghai 200032,China [3]School of Chemical Science and Technology,Yunnan University,Kunming 650091,China

出　　处：《Chinese Chemical Letters》2022年第10期4695-4699,共5页中国化学快报（英文版）

基　　金：supported by National Key R&D Program of China(No.2018YFA0507501);the National Science Foundation for Distinguished Young Scholars of China(No.21425518);the National Natural Science Foundation of China(Nos.22074019,22004017);Shanghai Sailing Program(No.20YF1405300).

摘　　要：Both glycosylation and phosphorylation exert crucial rule in multitudinous biological processes.For in-depth profiling of glycosylation and phosphorylation,a magnetic metal oxide is effectively coupled with inherently hydrophilic mesoporous channels(denoted as Fe_(3)O_(4)@TiO_(2)@mSiO_(2)-TSG).Based on the mechanism of hydrophilic interaction liquid chromatography(HILIC)and metal oxide affinity chromatography(MOAC),the Fe_(3)O_(4)@TiO_(2)@mSiO_(2)-TSG nanomaterial shows high capacity for simultaneously enriching glycopeptides and phosphopeptides.With human saliva collected in successive four days as practical biological sample,endogenous glycopeptides and phosphopeptides are efficiently enriched.Further gene ontology analysis reveals that the identified endogenous glycopeptides and phosphopeptides participate in diverse molecular functions and biological processes.This strategy is anticipated to promote variation analysis of salivary post-translational modifications.

关 键 词：Mesoporous material Hydrophilic interaction liquid chromatography Metal oxide affinity chromatography Human saliva Endogenous phosphopeptides Endogenous glycopeptides 

分 类 号：R318[医药卫生—生物医学工程]