不同占空比低强度诊断超声联合微泡对大鼠乏血供肿瘤血流灌注影响的实验研究  

Effect of low intensity diagnostic ultrasound with different duty cycle combined with microbubbles on blood perfusion of tumor with lack of blood supply in rats:a experimental study

作　　者：姚雷 杨国良 殷佳蓓 张毅 罗婷婷 唐家伟 唐君辉 刘政 YAO Lei;YANG Guoliang;YIN Jiabei;ZHANG Yi;LUO Tingting;TANG Jiawei;TANG Junhui;LIU Zheng(Department of Ultrasound,the Second Affiliated Hospital of Army Medical University,Chongqing 400037,China)

机构地区：[1]陆军军医大学第二附属医院超声科,重庆市400037

出　　处：《临床超声医学杂志》2022年第8期561-566,共6页Journal of Clinical Ultrasound in Medicine

基　　金：国家重大科研仪器研制项目(82127804)。

摘　　要：目的探讨不同占空比低强度诊断超声联合微泡对大鼠乏血供肿瘤血流灌注的影响。方法选取Wistar大鼠45只,于右侧背部皮下建立大鼠C6胶质细胞瘤模型,根据不同脉冲时间及间隔时间随机分为5组,每组各9只:A组,脉冲时间5 s,间隔时间1 s;B组,脉冲时间1 s,间隔时间1 s;C组,脉冲时间1 s,间隔时间5 s;D组,脉冲时间5 s,间隔时间5 s;E组,超声假照;各组对应的治疗脉冲占空比分别为0.167%、0.100%、0.033%、0.100%和0。每组荷瘤大鼠经超声联合微泡治疗10 min,于治疗前和治疗后即刻分别应用超声造影检查获取时间-强度曲线,比较各组治疗前后峰值强度(PI)、曲线下面积(AUC);分析各组超声造影图像进行超声造影视觉评分,并对其进行比较。各组随机选取3只大鼠取肿瘤组织行HE染色,观察治疗后病理学改变。结果A、B组治疗后AUC增加,与治疗前比较差异均有统计学意义(均P<0.05);各组治疗后PI及C、D、E组AUC与治疗前比较差异均无统计学意义。A、B组治疗后超声造影视觉评分均较高,与C、D、E组比较差异均有统计学意义(均P<0.05),余组间比较差异均无统计学意义。病理图示:A、B组可见较明显的血管扩张,A组部分血管周围组织内可见少量红细胞渗出,C、D、E组血管扩张均不明显。结论脉冲时间5 s、间隔时间1 s、占空比0.167%及脉冲时间1 s、间隔时间1 s、占空比0.100%的低强度诊断超声联合微泡可增强大鼠乏血供肿瘤血流灌注,后者为相对安全的超声治疗声学参数。Objective To investigate the effect of low intensity diagnostic ultrasound with different duty cycle combined with microbubbles on blood perfusion of tumor with lack of blood supply in rats.Methods Forty-five Wistar rats were selected to establish a rat C6 glioma model subcutaneously on the right back.According to different pulse time and interval time,they were randomly divided into 5 groups with 9 rats in each group:Group A,pulse time 5 s,interval time 1 s,Group B,pulse time 1 s,interval time 1 s,Group C,pulse time 1 s,interval time 5 s,Group D,pulse time 5 s,interval time 5 s,Group E,sham exposure with ultrasound.The corresponding treatment pulse duty cycles of each group were 0.167%,0.100%,0.033%,0.100% and 0,respectively.Tumor bearing rats in each group were treated by ultrasound combined with microbubbles for 10 min.Contrast-enhanced ultrasound was performed before and after treatment immediately to obtain the time-intensity curve.The peak intensity(PI)and area under the curve(AUC)of each group were compared.The contrast-enhanced ultrasound images of each group were compared,and the contrast-enhanced ultrasound visual scores were analyzed.Three rats in each group were randomly selected,and the tumor tissues were stained with HE to observe the pathological changes after treatment.Results Compared with the pre-treatment,the AUC of Group A and Group B increased after treatment,and the differences were statistically significan(t all P<0.05).There were no statistically significant changes in PI of each group and in AUC of Group C,Group D and Group E after treatment compared with pre-treatment.Compared with Group C,Group D and Group E,the contrast-enhanced ultrasound visual scores in Group A and Group B were higher,the differences were statistically significant(all P<0.05),while there were no significant differences among the other groups.The pathological graphic showed that obvious vasodilation was observed in Group A and Group B,a small amount of red blood cell exudation was observed in some perivascular tissue

关 键 词：超声辐照 微泡 空化效应 占空比 肿瘤 血流灌注 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学]