中性电解水对白色葡萄球菌的消毒效果及机制研究  

Disinfection effect and mechanism of neutral electrolyzed water on Staphylococcus albus

作　　者：黄珊珊 林秋莲 辛凡 温烯楠 阮国洪[1] Huang Shanshan;Lin Qiulian;Xin Fan;Wen Xinan;Ruan Guohong(School of Publish Health,Fujian Medical University,Fuzhou 350000,China)

机构地区：[1]福建医科大学公共卫生学院,福州350000

出　　处：《卫生研究》2022年第4期638-644,共7页Journal of Hygiene Research

摘　　要：目的评价中性电解水(neutral electrolyzed water,NEW)对白色葡萄球菌(Staphylococcus albus)的消毒效果,并分析其作用机制。方法通过悬液定量杀菌试验观察NEW的杀菌效果,测定细菌内容物泄露情况及相关酶活性,通过十二烷基磺酸钠聚丙烯酰胺凝胶电泳及琼脂糖凝胶电泳观察细菌蛋白及基因组DNA的破坏情况,分析NEW的作用机制。结果NEW作用1 min即可将悬液中的白色葡萄球菌(2.07×10^(7)CFU/mL)降至检出限以下(<10 CFU/mL),杀灭率>99.999%,杀灭对数值>5。NEW可破坏白色葡萄球菌细胞膜完整性,造成细胞内Ca^(2+)、K^(+)、蛋白质以及核酸泄露,引起超氧化物歧化酶、过氧化物酶、过氧化氢酶等酶活性发生改变并破坏蛋白质及基因组DNA。结论NEW可有效杀灭悬液中的白色葡萄球菌,其机制涉及NEW破坏细胞膜、菌体蛋白及核酸,以及改变酶活性。OBJECTIVE To evaluate the disinfection efficacy and mechanism of neutral electrolyzed water(NEW)on Staphylococcus albus.METHODS The bactericidal effect of NEW was observed through suspension quantitative bactericidal test.In addition,to analyze the mechanism of NEW,the leakage of bacterial contents and enzyme activity were tested,and the damage of bacterial protein and DNA were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and agarose gel electrophoresis.RESULTS After treated with NEW for 1 min,staphylococcus albus in suspension(2.07×10^(7) CFU/mL)were reduced to below the detection limit(<10 CFU/mL),with killing rate>99.999% and killing log value>5.NEW could destroy the integrity of the cell membrane,resulting in the leakage of Ca^(2+),K^(+),protein and nucleic acid,and change the activity of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and other enzymes,it could also damage protein and DNA.CONCLUSION NEW can effectively kill Staphylococcus albus in suspension,and its mechanism involves the destruction of cell membrane,bacterial protein and nucleic acid,and the change of enzyme activity.

关 键 词：中性电解水 白色葡萄球菌 消毒 

分 类 号：R123[医药卫生—环境卫生学]