LncRNA-NBAT1介导miR-21/PTEN轴调控子宫内膜癌细胞侵袭转移的机制研究  

The regulation mechanism of LncRNA-NBAT1 mediated miR-21/PTEN axis in the invasion and metastasis of endometrial cancer cells

作　　者：田春花 陈冲 吴阳 马钊 马鸿云[1] TIAN Chunhua;CHEN Chong;WU Yang;MA Zhao;MA Hongyun(People's Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China)

机构地区：[1]宁夏回族自治区人民医院,宁夏银川750002

出　　处：《宁夏医学杂志》2022年第8期676-679,共4页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(2020AAC03336);宁夏回族自治区卫生健康系统科研课题[宁卫办发(2020)256号];宁夏回族自治区人民医院培育振兴科研项目(202109)。

摘　　要：目的探究长链非编码RNA-NBAT1是否可以通过调控LncRNA-NBAT1-miR21/PTEN轴来调控子宫内膜癌细胞的生长。方法构建LncRNA-NBAT1过表达及空载载体,并合成miR-21-5p mimic空载(NC)miR-21-5p mimic(mimic)后,分别转染两款人子宫内膜癌细胞株:HEC-1A和Ishikawa。转染24 h后,采用荧光定量PCR(qPCR)检测方法检测HEC-1A和Ishikawa细胞中LncRNA-NBAT1mRNA、miR-21-5pmRNA表达;采用Western blot检测PTEN蛋白表达水平。结果LncRNA-NBAT1、PTEN对人子宫内膜癌细胞增殖具有抑制作用,miR-21-5p对人子宫内膜癌细胞增殖具有促进作用;LncRNA-NBAT1可上调PTEN的mRNA表达,下调miR-21-5p的mRNA表达。结论LncRNA-NBAT1可通过调控miR-21/PTEN轴来有效抑制调控人子宫内膜癌细胞的增长。Objective To explore whether LncRNA-NBAT1 can regulate the growth of endometrial cancer cells by regulating the miRLncRNA NBAT1-21/PTEN axis.Methods The overexpression and empty vector of LncRNA-NBAT1 were constructed,and miR-21-5 p mimic empty(NC)miR-21-5 p mimic(mimic)was synthesized,and then transfected into human endometrial cancer cell HEC-1 A and Ishikawa lines respectively.After 24 hours,the expression of LncRNA-NBAT1 m RNA and miR-21-5 pmRNA in HEC-1 A and Ishikawa cells were detected by fluorescence quantitative PCR(qPCR).The protein expression level of PTEN was detected by Western blot.Results LncRNA-NBAT1 and PTEN inhibited the proliferation of human endometrial cancer cells,while miR-21-5 p promoted the proliferation of human endometrial cancer cells.LncRNA-NBAT1 up-regulated the expression of PTENmRNA and down-regulated the expression of miR-21-5 p.Conclusion LncRNA-NBAT1 can effectively regulate the growth of human endometrial cancer cells by the miR-21/PTEN axis.

关 键 词：miR-21-5p 长链非编码RNA-NBAT1 PTEN 人子宫内膜癌细胞株 

分 类 号：R614[医药卫生—麻醉学]