红麻HcMYB3基因克隆和表达分析  

Gene Cloning and Expression Analysis of HcMYB3 in Kenaf

作　　者：杨大为 邓勇[1] 张超 栾明宝[1] 黄思齐[1] 李建军[1] 常丽 潘根 唐慧娟[1] 李德芳[1] YANG Dawei;DENG Yong;ZHANG Chao;LUAN Mingbao;HUANG Siqi;LI Jianjun;CHANG Li;PAN Gen;TANG Huijuan;LI Defang(Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences/Key Laboratory of Hemp Biology and Processing,Ministry of Agriculture,Changsha 410205,Hunan,China)

机构地区：[1]中国农业科学院麻类研究所/农业农村部麻类生物学与加工重点实验室,湖南长沙410205

出　　处：《中国麻业科学》2022年第4期201-210,共10页Plant Fiber Sciences in China

基　　金：财政部和农业农村部:国家麻类产业技术体系资助(CARS-16-E5);中国农业科学院科技创新工程:一年生麻类遗传改良团队(ASTIP-IBFC03);中央级公益性科研院所基本科研业务费专项(1610242021002)资助。

摘　　要：为探究HcMYB3基因在红麻中的潜在功能和盐胁迫响应规律,研究以红麻品种“中红麻21号”为试验材料,对HcMYB3基因进行同源克隆,并进行生信分析以及盐胁迫下时空表达模式分析。结果表明,HcMYB3基因包含675 bp的开放阅读框,编码224个氨基酸,编码的蛋白质为酸性,具有不稳定、亲水性、不跨膜、不含信号肽的特性,二级结构以无规则卷曲为主,与木槿Hs MYB3亲缘关系最近。HcMYB3基因在中低浓度盐(50、150 mmol/L NaCl)胁迫下显著上调,高浓度盐(250 mmol/L NaCl)胁迫下随时间呈“降—增—降”的趋势。研究为红麻耐盐调控网络的研究提供了理论基础。To explore the potential function of Hc MYB3 gene in kenaf and its mechanism response to stress,Hc MYB3 gene isolated via homologous cloning method was performed in kenaf cultivar"Zhonghong No.21",followed by bioinformatics analysis and spatio-temporal expression pattern analysis under salt stress.The results showed that the Hc MYB3 gene contained an open reading frame of 675 bp,encoding 224 amino acids,and the secondary structure was dominated by random coil,and the encoded proteins had the characteristics of acidity,unstable,hydrophilicity,non-transmembrane,signal-free peptides.The Hc MYB3 gene was significantly upregulated under the stress of medium and low concentra-tion salts(50 mmol/L,150 mmol/L NaCl),and the trend of"decrease-increase-decrease"was shown over time under high salt concentration of 250 mmol/L NaCl.This study provided theoretical basis for the study of salt-tolerant regulation network of kenaf.

关 键 词：红麻 HcMYB3 克隆 表达分析 盐胁迫 

分 类 号：S563.5[农业科学—作物学]