基于毛花猕猴桃基因组的性别相关SSR分子标记的开发  

Development of sex-related SSR molecular markers based on the genome of Actinidia eriantha

作　　者：刘嘉艺 岳俊阳 刘永胜 LIU Jiayi;YUE Junyang;LIU Yongsheng(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601

出　　处：《合肥工业大学学报（自然科学版）》2022年第8期1135-1138,1146,共5页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31471157)。

摘　　要：猕猴桃作为雌雄异株植物,其早期性别鉴定对于育种和增加其经济价值有着重要意义。文章通过开发毛花猕猴桃性别相关的简单重复序列(simple sequence repeat, SSR)分子标记,以期能在早期鉴定出毛花猕猴桃雌雄植株,提高毛花猕猴桃资源利用率。利用MISA工具从毛花猕猴桃雌雄基因组的29条染色体筛选获得381 250个SSR位点,并对SSR位点的数量与分布特征进行统计分析。设计合成150对引物,利用毛花猕猴桃基因组DNA作为模板,采用普通聚合酶链式反应(polymerase chain reaction, PCR)扩增的方法以及聚丙烯酰胺凝胶电泳(PAGE)分离检测方法筛选出具有性别差异扩增片段的SSR引物。其中序号为Primer000181的引物表现为雄性特异。在毛花猕猴桃5个雄株、2个雌株样本中进行验证,其表现均与前文一致。结果表明利用毛花猕猴桃基因组开发性别鉴定相关SSR分子标记具有可行性,并筛选出1对可以鉴定毛花猕猴桃雌雄植株的引物,能够在毛花猕猴桃生长早期可靠、快速地鉴定其性别。Kiwifruit is a dioecious plant,and its early sex identification is of great significance for breeding and increasing its economic value.In order to identify male and female plants of Actinidia eriantha in early stage and improve the utilization rate of A.eriantha resources,the sex-related simple sequence repeat(SSR)molecular markers were developed.The MISA tool was used to screen 381250 SSR loci from 29 chromosomes of the male and female genomes of A.eriantha,and the number and distribution characteristics of SSR loci were statistically analyzed.The 150 pairs of primers were designed and synthesized.The genomic DNA of A.eriantha was used as template,and the SSR primers with sex-specific amplified fragments were screened by polymerase chain reaction(PCR)amplification and polyacrylamide gel electrophoresis(PAGE).The primer with serial number Primer000181 is male-specific.It was verified in samples of five male plants and two female plants of A.eriantha,and their performance was consistent with the previous results.This study confirms the feasibility of using the A.eriantha genome to develop SSR molecular markers related to sex identification,and screens out a pair of primers that can identify the male and female plants of A.eriantha.The sex of A.eriantha can be reliably and quickly identified in the early growth stage.

关 键 词：毛花猕猴桃 基因组 简单重复序列(SSR) 性别鉴定 雄性特异 

分 类 号：Q812[生物学—生物工程]