盐胁迫下白花前胡香豆素合成途径关键酶基因生物信息学分析  

Bioinformatics Analysis of Key Enzyme Genes Involved in Coumarin Biosynthetic Pathway of Peucedanum praeruptorum Dunn Under Salt Stress

作　　者：李姚碧琪 陆海波[1,2] 贾彬 殷齐慧 张艳梅 韩邦兴 欧金梅 LI Yao-bi-qi;LU Hai-bo;JIA Bin;YIN Qi-hui;ZHANG Yan-mei;HAN Bang-xing;OU Jin-mei(Anhui University of Chinese Medicine,Anhui Hefei 230012,China;West Anhui University,Anhui Hefei 237012,China;Anhui Agricultural University,Anhui Hefei 230038,China)

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]皖西学院,安徽六安237012 [3]安徽农业大学,安徽合肥230038

出　　处：《安徽中医药大学学报》2022年第4期87-91,共5页Journal of Anhui University of Chinese Medicine

基　　金：安徽省杰出青年基金(1808085J17);现代农业产业技术体系(CARS-21)。

摘　　要：目的系统研究白花前胡香豆素合成途径关键酶基因的蛋白结构特征,并明确关键酶基因在盐胁迫下的表达模式。方法通过白花前胡前期转录组测序,确定前胡香豆素生物合成关键酶基因的序列,利用蛋白质结构分析预测软件对关键酶蛋白进行系统预测分析,并通过qRT-PCR分析盐胁迫下关键酶基因的变化规律。结果关键酶基因编码蛋白都有着各自特殊的保守结构域和三级结构;0.15 mol/L NaCl处理4 h对PAL和4CL基因的表达水平影响最高,比对照组上调1倍;而COMT-S和GXM在不同处理时间下,基因表达水平均明显下降。结论受盐胁迫后上调表达的香豆素合成途径关键酶基因,可以提高植物对逆境胁迫的耐受性。Objective To systematically investigate the protein structure characteristics of key enzyme genes involved in the coumarin biosynthetic pathway of Peucedanum praeruptorum Dunn,and to clarify the expression pattern of key enzyme genes under salt stress.Methods Transcriptome sequencing was performed for Peucedanum praeruptorum Dunn to determine the sequence of the key enzyme genes involved in coumarin biosynthesis,and the protein structure analysis and prediction software was used to perform a systematical predictive analysis of key enzyme proteins.qRT-PCR was used to analyze the changes in key enzyme genes under salt stress.Results The proteins encoded by the key enzyme genes had their own special conserved domains and tertiary structure.Treatment with 0.15 mol/L NaCl for 4 hours had the greatest influence on the expression levels of the PAL and 4 CL genes,which were one time higher than those in the control group.There were significant reductions in the expression levels of the COMT-S and GXM genes after treatment for different times.Conclusion The expression of key enzyme genes involved in the coumarin biosynthesis pathway is upregulated after salt stress,which can improve the tolerance of plants to adversity stress.

关 键 词：白花前胡 香豆素 生物合成途径 盐胁迫 关键酶 

分 类 号：R917[医药卫生—药物分析学]