基于代谢组学技术研究枸杞多糖对雏鸡免疫抑制的拮抗机制  

Antagonistic Mechanism of Lycium barbarum Polysaccharides on Immunosuppression of Chicks Based on Metabonomics Technology

作　　者：王建东 唐玉林 王敏 张宝锁 杨富强 高海慧 于洋 郭延生[2] 梁小军 WANG Jiandong;TANG Yulin;WANG Ming;ZHANG Baosuo;YANG Fuqiang;GAO Haihui;YU Yang;GUO Yansheng;LIANG Xiaojun(Institute of Animal Science,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China;Department of Animal Science and Technology,College of Agriculture,Ningxia University,Yinchuan 750021,China;Maternal and Child Health Care Hospital of Ningxia,Yinchuan 750001,China;Ningxia Shunbao Modern Agriculture Co.,Ltd.,Qingtongxia 751600,China)

机构地区：[1]宁夏农林科学院动物科学研究所,银川750002 [2]宁夏大学农学院,银川750021 [3]宁夏银川市妇幼保健院,银川750001 [4]宁夏顺宝现代农业股份有限公司,青铜峡751600

出　　处：《动物营养学报》2022年第8期5354-5363,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：宁夏自然科学重点基金项目(2020AAC02032)。

摘　　要：本试验旨在通过代谢组学技术研究枸杞多糖干预免疫抑制雏鸡后对脾脏代谢的影响,分析脾脏差异代谢物及筛选生物标志物,为明确枸杞多糖拮抗雏鸡免疫抑制机制提供参考依据。将120羽7日龄海兰褐蛋鸡随机分成3个组,即空白组(NC组)、环磷酰胺组(CY组)及枸杞多糖组(CYLbGp组),每组40羽,除NC组外,其余2组连续3 d按照80 mg/(kg·d)的剂量胸肌注射环磷酰胺溶液(以生理盐水为溶剂)进行造模,NC组胸肌注射等体积的生理盐水,造模后在CYLbGp组饮水中加入5 mg/(kg·d)枸杞多糖,持续30 d。在CYLbGp组给药结束后第1天,每组选取6只雏鸡采集脾脏,采用基于超高效液相色谱串联三重四级杆飞行时间质谱(UHPLC-Triple-TOF-MS)代谢组学技术对其脾脏进行检测。采用主成分分析(PCA)法、偏最小二乘判别分析(PLS-DA)法和正交偏最小二乘判别分析(OPLS-DA)法对3组脾脏代谢轮廓进行分析,采用MetaboAnalyst 5.0进行单变量ROC曲线分析,筛选生物标志物。结果显示:3组脾脏代谢轮廓发生明显改变,14种差异代谢物被确认为生物标志物[变量投影重要度(VIP)>1,P<0.05],其中13种差异代谢物经枸杞多糖干预后含量显著下调(P<0.05),1种差异代谢物含量显著上调(P<0.05)。上述研究结果提示,枸杞多糖拮抗雏鸡免疫抑制的作用机制可能与纠正脂质代谢功能紊乱、抗氧化应激和改善脾脏功能有关。This experiment was conducted to study the effects of Lycium barbarum polysaccharide on spleen metabolism of immunosuppressive chicks,analyze spleen differential metabolites and screen biomarkers based on metabonomics technology,in order to provide reference basis for Lycium barbarum polysaccharide to antagonize immunosuppression in chicks.One hundred and twenty 7-day-old Hy-line brown chicks were randomly divided into 3 groups,including blank group(NC group),cyclophosphamide group(CY group)and wolfberry polysaccharide group(CYLbGp group),with 40 chicks in each group.Except for the NC group,chicks in the other 2 groups were injected with cyclophosphamide solution(with normal saline as solvent)into the chest muscle at a dose of 80 mg/(kg·d)for 3 consecutive days for modeling,and chicks in the NC group were injected with an equal volume of normal saline into the chest muscle.After modeling,Lycium barbarum polysaccharide with the dose of 5 mg/(kg·d)was added to the drinking water of the CYLbGp group for 30consecutive days.The spleen was collected from six chicks in each group on day 1 after the end of administration of CYLbGp group.Ultra performance liquid chromatography tandem triple quadrupole time-of-flight mass spectrometry(UHPLC-Triple-TOF-MS)metabolomics technology was used to detect the spleen.Principal component analysis(PCA)method,partial least squares discriminant analysis(PLS-DA)method and orthogonal partial least squares discriminant(OPLS-DA)method were used to analyze the metabolic profiles,and Metaboanalyst 5.0 was used to analyze univariate ROC curves screening for biomarkers.The results showed that the metabolic profile of the spleen in the 3 groups obviously changed,and 14 differential metabolites were confirmed as biomarkers[variable projected importance(VIP)>1,P<0.05],of which 13 metabolites were significantly down-regulated after the intervention of Lycium barbarum polysaccharide(P<0.05),and one metabolite was significantly up-regulated(P<0.05).The above findings suggest that the mechanism of L

关 键 词：枸杞多糖 环磷酰胺 免疫抑制 脾脏 代谢组学 

分 类 号：S831[农业科学—畜牧学]