蚕豆始荚节位与始荚高度性状的遗传分析及QTL定位  

Genetic Analysis and QTL Mapping of Initial Pod Node Position and Initial Pod Height in Vicia faba L.

作　　者：吴小燕 周仙莉 张红岩 彭小星 范惠玲 滕长才[2] 刘玉皎 WU Xiaoyan;ZHOU Xianli;ZHANG Hongyan;PENG Xiaoxing;FAN Huiling;TENG Changcai;LIU Yujiao(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Academy of Agricultural and Forestry Sciences,Xining 810016,China;State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学农牧学院,西宁810016 [2]青海省农林科学院,西宁810016 [3]青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《四川农业大学学报》2022年第4期498-504,600,共8页Journal of Sichuan Agricultural University

基　　金：青藏高原种质资源研究与利用实验室项目(2022-ZJ-Y01);国家现代农业产业技术体系(CARS-08)。

摘　　要：【目的】始荚节位与始荚高度是蚕豆机械化生产的重要性状,开发相关的分子标记,为适宜机械化生产的蚕豆种质创新与新品种选育提供科学依据。【方法】以青蚕19号为母本,青海13号为父本进行杂交构建F_(2)群体,采用植物数量性状“主基因+多基因”混合遗传模型分析始荚性状的遗传特性,利用SSR标记对F_(2)群体的始荚节位与始荚高度进行QTL定位。【结果】蚕豆始荚节位符合2对加性-显性-上位性主基因模型(2MG-ADI)遗传模型,始荚高度性状符合2对加性-显性主基因模型(2MG-AD)遗传模型。在1596对蚕豆SSR引物中筛选出79对在群体中表现多态性的引物,构建了青蚕19号/青海13号F_(2)群体的遗传图谱,该图谱包含6个连锁群,图谱总长度为1804.59 cM,标记间平均距离为22.84 cM,各连锁群长度介于26.34~1389.89 cM之间,连锁群的标记数量介于2~54个。共定位到5个始荚节位相关的QTLs和8个始荚高度相关的QTLs。【结论】蚕豆的始荚节位与始荚高度分别由两对主基因控制,并挖掘到5个与始荚节位相关、8个与始荚高度相关的分子标记,为定向选育适宜始荚节位和始荚高度的优良品种提供理论依据。【Objective】The initial pod node position and initial pod height are important mechanized production traits in faba bean.The development of relevant molecular markers for assisted breeding provides a basis for the innovation of faba bean germplasm suitable for mechanized production and new variety breeding.【Method】The study,a F_(2) population was constructed by crossing Qingchan 19(female parent)and Qinghai 13(male parent).The genetic characteristics of the initial pod traits were analyzed by using the mixed genetic model of major gene+polygene,and the QTLloci for the initial pod node and height of F_(2) population were located by SSR markers.【Result】The results showed that genetic basis for initial pod position trait of Vicia faba was accorded with two pairs of additive-dominant-epistatic major gene model(2MG-ADI),and that of the initial pod height trait was accorded with two pairs of additive dominant major gene model(2MG-AD).1596 pairs of faba bean SSR primer,were used to screen the F_(2) population and 79 pairs of primer showed polymorphic;Furthermore,the genetic linkage map of Qingcan 19/Qinghai 13 F_(2) population was constructed.This map contained 6 linkage groups,the total length of the map was 1804.59 cM,the average distance between two markers was 22.84 cM,the length of each link‐age group was 26.34~1389.89 cM,and the number of markers in the linkage group was 2~54.Based on the map,5 QTL loci related to initial pod node and 8 QTL loci related to pod height.【Conclusion】The initial pod node position and initial pod height of faba bean are controlled by two pairs of main genes,and five molecular markers related to the initial pod node position and eight molecular markers related to the initial pod height have been excavated,which provided a theoretical basis for directional selection of good varieties with suitable initial pod node position and initial pod height.

关 键 词：始荚节位 始荚高度 遗传分析 QTL分析 

分 类 号：S643.6[农业科学—蔬菜学]