基于谱效关系的荆芥穗药效部位治疗结肠癌药效质量标志物研究  

Screening of Pharmacodynamic Substances for the Treatment of Colon Cancer in Schizonepeta Spike Flavonoids Based on Pharmacodynamic Quality Markers

作　　者：贾梦楠 王帅[1,2] 李天娇 包永睿[1,2] 孟宪生 Jia Mengnan;Wang Shuai;Li Tianjiao;Bao Yongrui;Meng Xiansheng(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Engineering Research Center of Traditional Chinese Medicine,Liaoning Engineering Laboratory of Modern Traditional Chinese Medicine,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药多维分析专业技术创新中心,辽宁省现代中药研究工程实验室,辽宁大连116600

出　　处：《亚太传统医药》2022年第8期33-38,共6页Asia-Pacific Traditional Medicine

基　　金：辽宁省自然科学基金(2019-ZD-0946)。

摘　　要：目的:基于灰色关联分析法及偏最小二乘回归法预测荆芥穗药效部位体外治疗结肠癌的药效质量标志物,为其质量控制提供参考依据。方法:采用HPLC法建立不同产地荆芥穗化学轮廓谱,且进行对照品比对;采用AO/EB染色法及CCK-8法,开展荆芥穗治人肠癌HT-29细胞的药效学研究;采用灰色关联及偏最小二乘法,基于化学轮廓谱与抗肿瘤活性的关联,筛选荆芥穗治疗结肠癌的药效质量标志物。结果:10批不同产地荆芥穗药效部位相似度良好,相似度均>0.96。不同产地荆芥穗提取物均能明显抑制人肠癌HT-29细胞的增殖。灰色关联及偏最小二乘法分析结果显示,原儿茶醛、咖啡酸、对香豆酸、木犀草苷、橙皮苷、异绿原酸C、木犀草素、香叶木素与荆芥穗药效部位治疗结肠癌作用关联度值较高且呈正相关。结论:荆芥穗药效部位中的8种成分可以作为荆芥穗治疗结肠癌的潜在药效质量标志物。Objective:Based on grey correlation analysis and partial least squares regression method,the pharmacodynamic quality markers of schizonepeta panicle in vitro treatment of colon cancer were predicted to provide a reference for its quality control.Methods:HPLC method was used to establish the chemical profile of schizonepeta panicle from different producing areas.AO/EB staining and CCK-8 staining were used to study the pharmacodynamics of schizonepeta in the treatment of human colorectal cancer HT-29 cells.Grey correlation and partial least square method were used to screen the pharmacodynamic quality markers of schizonepeta in the treatment of colon cancer.Results:The similarity of medicinal parts of 10 batches of schizonepeta brassica paniculata from different producing areas was good,and the similarity was greater than 0.96.The extracts of schizonepeta from different habitats could inhibit the proliferation of HT-29 cells.The results of grey correlation and partial least square analysis showed that protocatechualdehyde,caffeic acid,p-maric acid,luteolin,hesperidin,isochlorogenic acid C,luteolin and geranitin were positively correlated with the therapeutic effect of schizonepeta schizonepeta ear on colon cancer.Conclusion:The 8 components in the effective part of schizonepeta spike could be used as potential effective quality markers in the treatment of colon cancer.

关 键 词：荆芥穗 结肠癌 药效质量标志物 谱效关系 高效液相色谱 

分 类 号：R285.5[医药卫生—中药学]