不同炮制方法对柿叶成分及止咳祛痰作用的影响  

Effects of Different Processing Methods on Components and Antitussive Effect of Diospyros Kaki Thumh.

作　　者：蒋馨 李健[4] 赖国校 李利群 周改莲[1,2,3] 郝二伟 Jiang Xin;Li Jian;Lai Guoxiao;Li Liqun;Zhou Gailian;Hao Erwei(Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues,Guangxi University of Chinese Medicine,Nanning 530200,China;Higher Vocational and Technical College of Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西中医药大学广西中药药效研究重点实验室,广西南宁530200 [3]广西中医药大学农作物废弃物功能成分协同创新中心,广西南宁530200 [4]广西中医药大学高等职业技术学院,广西南宁530001

出　　处：《亚太传统医药》2022年第8期39-43,共5页Asia-Pacific Traditional Medicine

基　　金：广西重点实验室建设项目(17-259-20)。

摘　　要：目的:比较柿叶不同炮制品有效成分含量的差异,柿叶蜜炙前后祛痰镇咳功效的影响。方法:运用紫外分光色谱法测定柿叶不同炮制品总黄酮含量,HPLC法测定不同柿叶炮制品芦丁、山柰酚、槲皮素含量;氨水致咳实验比较柿叶生品和蜜炙品止咳作用,测量小鼠气管酚红分泌量,比较柿叶生品和蜜炙品祛痰作用。结果:柿叶炮制品总黄酮、芦丁含量均低于生品,柿叶炒黄、酒炙后槲皮素、山柰酚含量高于生品,其他炮制品均低于生品。柿叶蜜炙品和生品均能止咳祛痰,但蜜炙品祛痰止咳功效优于生品。结论:柿叶炮制前后总黄酮、芦丁、槲皮素、山柰酚含量均发生变化;柿叶蜜炙后止咳祛痰功效增强。Objective:The effects of different processed products of Diospyros Kaki Thumh.on the content of active ingredients were compared,and the effects of Diospyros Kaki Thumh.treated with honey on the antitussive and expectorant activity effects were studied.Methods:The content of total flavonoids in Diospyros Kaki Thumh.of different processing methods was determined by ultraviolet spectrophotometry.The content of rutin,kaempferol and quercetin in different processing methods were determined by HPLC.The antitussive effect of Diospyros Kaki Thumh.treated with honey was studied.The processed Diospyros Kaki Thumh.expectorant activity was evaluate by determined the amount of tracheal phenol red secretion in mice.Results:The content of total flavonoids and rutin of Diospyros Kaki Thumh.after processing was lower than that of raw products.The content of quercetin and kaempferol in the Diospyros Kaki Thumh.treated with rice wine and fired was higher than that of raw products.The Diospyros Kaki Thumh.after processing by other methods were lower than the raw products.The Diospyros Kaki Thumh.treated with honey and the Diospyros Kaki Thumh.of the raw products can antitussive and expectorant activity,but the Diospyros Kaki Thumh.treated with honey is better than the raw products.Conclusion:The contents of total flavonoids,rutin,quercetin and kaempferol in different leaves were changed after different treatments.Diospyros Kaki Thumh.treated with honey can enhance antitussive and expectorant activity.

关 键 词：柿叶 炮制品 高效液相色谱 止咳祛痰 

分 类 号：R285.5[医药卫生—中药学]