HPLC-MS/MS法测定32%肟菌·咪鲜胺水乳剂有效成分含量  

Analysis of active ingredients in trifloxystrobin·prochloraz 32%EW by HPLC-MS/MS

作　　者：龚会琴 周亚 谭正莹 何钰 张冰梅 包娜 GONG Huiqin;ZHOU Ya;TAN Zhengying;HE Yu;ZHANG Bingmei;BAO Na(Guizhou Academy of Testing and Analysis,Guiyang 550014,China;Guizhou Testing Technology Research and Application Center,Guiyang 550014,China)

机构地区：[1]贵州省分析测试研究院,贵阳550014 [2]贵州省检测技术研究应用中心,贵阳550014

出　　处：《世界农药》2022年第8期56-60,共5页World Pesticide

基　　金：贵州省高层次创新型人才培养项目(千层次);黔科院J字[2021]41号;贵州科学院青年基金项目(黔科院J字[2021]45号)。

摘　　要：采用HPLC-MS/MS建立一种同时检测32%肟菌·咪鲜胺水乳剂中有效成分含量的分析方法。采用Poroshell 120 EC-C_(18)色谱柱和MSD检测器,以甲醇-0.1%甲酸水溶液为流动相进行梯度洗脱,对试样中的肟菌酯和咪鲜胺进行分离和定量测定。该分析方法中肟菌酯和咪鲜胺线性相关系数分别为0.9991和0.9996,变异系数分别为0.66%和0.57%,平均回收率分别为101.01%、100.50%。该分析方法操作简单、快速准确,是检测32%肟菌·咪鲜胺水乳剂中有效成分含量的理想方法。The analytical method for determining trifloxystrobin and prochloraz in the product trifloxystrobin·prochloraz 32%EW was established by HPLC-MS/MS.The separation and determination of trifloxystrobin and prochloraz were conducted with Poroshell 120 EC-C_(18) column and MSD,methanol and 0.1%formic acid aqueous solution as the mobile phase for gradient elution.In this analytical method,the linear correlation coefficients of trifloxystrobin and prochloraz were 0.9991 and 0.9996,the coefficients of variation were 0.66%and 0.57%,and the average recoveries were 101.01%and 100.50%,respectively.This method is an ideal method for the determination of trifloxystrobin·prochloraz 32%EW with the characteristics of simpleness,fastness and accuracy.

关 键 词：肟菌酯 咪鲜胺 HPLC-MS/MS 

分 类 号：TQ450.7[化学工程—农药化工]