不同干扰强度对华北落叶松-云杉混交林土壤细菌群落组成及多样性的影响  

Effects of different interference intensity on soil bacterial communities composition and diversity in Larix principisrupprechtii and Picea acperata mingled forest

作　　者：闫海冰 李璟 陈颂 周朵朵 Yan Haibing;Li Jing;Chen Song;Zhou Duoduo(Department of Environmental and Safety Engineering,Taiyuan Institute of Technology,Taiyuan 030008,China;Ecology and Nature Conservation Institute,CAF,Beijing 100000,China;College of Forestry,Shanxi Agricultural University,Jinzhong 030801,China)

机构地区：[1]太原工业学院环境与安全工程系,山西太原030008 [2]中国林业科学研究院森林生态环境与自然保护研究所,北京100000 [3]山西农业大学林学院,山西晋中030801

出　　处：《山西农业大学学报（自然科学版）》2022年第4期85-95,共11页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31670630;31470631);山西省自然科学基金(201901D111224)。

摘　　要：[目的]探测干扰对华北亚高山森林土壤细菌群落组成及多样性的影响及其驱动机制。[方法]高通量测序技术分析关帝山庞泉沟自然保护区华北落叶松⁃云杉混交林不同干扰等级(未干扰、Ⅰ级干扰、Ⅱ级干扰)林分土壤细菌多样性及丰度,方差分析及NMDS分析不同干扰强度下细菌群落结构的差异,RDA分析土壤理化性质对细菌群落分布的相对贡献。[结果]不同干扰等级的森林土壤样品细菌群落丰度在门水平以酸杆菌门呈显著性差异且Ⅰ级干扰样地(S1)>Ⅱ级干扰样地(S2)>未干扰样地(N),在属水平以RB41属(S1>N>S2)、MND1属(S2>S1>N)差异显著。未干扰样地(N)土壤细菌物种丰富度和均匀度较低,随干扰加大,Ⅰ级干扰样地(S1)各项Alpha多样性指数均显著提高。随干扰强度由Ⅰ级增至Ⅱ级,对应干扰样地S2各项指数较S1有所下降。适度干扰可提高土壤细菌多样性,过轻或过重的干扰都将使土壤细菌多样性降低。对3个样地细菌群落的差异分析表明,随干扰强度增大,干扰对细菌群落结构的影响变得显著。与未干扰样地相比,受干扰样地(Ⅰ、Ⅱ级干扰样地)土壤温度显著下降,土壤有机质、有效氮、有效钾、有效锌随干扰等级的增加呈现先减后增的变化趋势。这些土壤因子对土壤细菌群落(门水平和属水平)结构影响显著。土壤理化性质对细菌群落在门水平的模型解释量为63.13%,属水平的解释量为60.66%。其中有效氮对土壤细菌群落结构影响最大,可解释15.61%(门水平)和18.56%(属水平)的土壤细菌群落结构。[结论]不同强度干扰通过改变土壤理化性质显著影响了华北亚高山森林土壤细菌群落组成及多样性结构。[Objective]The present study was to investigate the effects of disturbance on soil bacterial community composition and diversity in subalpine forest in north China and its driving mechanism.[Methods]High-throughput sequencing technology was employed to study the soil bacterial diversity and abundance in different disturbance levels(undisturbed,level I interference,and level Ⅱ interference)in Pangquangou Nature Reserve,Guandi Mountain in Larix principis-rupprechtii and Picea acperata mingled forest. Using one-way ANOVA and non-metric multi-dimensional scaling,we analyzed the differences of bacterial community structure under different interference intensities. Using redundancy analysis,we analyzed the relative contribution of soil physical and chemical properties to the distribution of bacterial communities.[Results]The abundance of bacterial community in forest soil samples with different interference levels was significantly different at phylum level,and the acidobacteria phyla were significantly different and the level Ⅰ interference plot(S1)>Level Ⅱ interference plot(S2)> the undisturbed plot(N). At the genus level,there were significant differences in genus RB41(S1>N>S2)and MND1(S2>S1>N). The richness and evenness of soil bacterial species in no interference plot(N)were low,and the Alpha diversity indices of soil bacterial species in level Ⅰ interference plot(S1)were significantly increased with the increase of interference frequency.When the interference intensity increased from level Ⅰ level to level Ⅱ,the indices of the corresponding interference plot(S2)decreased compared to S1. Moderate interference could improve soil bacterial diversity,while too light or heavy interference all reduced soil bacterial diversity. The difference analysis of bacterial communities in the three plots showed that the influence of interference on bacterial community structure became significant with the increase of disturbance intensity. Compared with no interference plots,soil temperature in disturbed plots(level �

关 键 词：亚高山针叶林 干扰 细菌群落结构 土壤理化因子 

分 类 号：S791.2[农业科学—林木遗传育种]