蒙古沙冬青AmRD22基因的克隆及耐逆功能研究  

Cloning of AmRD22 Gene from Ammopiptanthus mongolicus and Its Function in Stress Tolerance

作　　者：张敏 王学峰[1] 马利 刘佳杰[1] 张慧玲[1] 王茅雁[1] ZHANG Min;WANG Xuefeng;MA Li;LIU Jiajie;ZHANG Huiling;WANG Maoyan(College of Life Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018

出　　处：《西北植物学报》2022年第7期1083-1092,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家转基因生物新品种培育重大专项(2009ZX08009-019B/003);国家自然科学基金(31560299);内蒙古自治区自然科学基金(2012ZD02)。

摘　　要：脱水应答蛋白22(RD22)属于植物特有的BURP蛋白家族中的一个亚族,与耐逆性关系密切。该研究从中国西北荒漠区特有的强耐逆植物蒙古沙冬青克隆到一个RD22基因(AmRD22)的全长cDNA,并对其编码蛋白、表达模式和耐逆功能进行了研究。结果表明:(1)AmRD22蛋白(360 aa)的初级结构中含有RD22亚族共有的4个结构域,预测其定位于细胞壁;在功能已知的RD22蛋白中,AmRD22与大豆GmRD22的进化关系最近。(2)在室内培养的蒙古沙冬青幼苗中,AmRD22的表达受失水、高盐、低温和ABA胁迫的诱导显著上调,其中失水和低温胁迫诱导其上调幅度较大;在野外生长的蒙古沙冬青植株嫩叶中,其表达量从中秋至隆冬远高于其他季节。(3)转AmRD22基因拟南芥的耐盐性显著提高且Na^(+)含量降低,其耐旱性也有较明显的改善且在种子萌发早期对外源ABA的敏感性降低,但耐冷性和耐冻性无明显变化。The responsive to dehydration protein 22 s(RD22 s)constitute a subfamily of the plant-specific BURP domain-containing protein family,which are closely related to stress tolerance of plants.In this study,the full-length cDNA for a RD22 gene(AmRD22)was cloned from Ammopiptanthus mongolicus,a plant endemic to desert areas in Northwest China and with strong tolerance to abiotic stresses.Moreover,its coding protein,expression pattern and biological function in stress tolerance were analyzed.The results showed that:(1)the AmRD22 protein(360 aa)contains all four domains common to the RD22 subfamily and it most probably locates in cell wall.Among the reported RD22 proteins with known biological function,AmRD22 has the closest phylogenetic relationship with soybean GmRD22.(2)In laboratory-cultured A.mongolicus seedlings,the expression of AmRD22 was induced by dehydration,high salinity,cold and exogenous ABA,and the induction was relatively high under the dehydration and cold stresses.In young leaves of A.mongolicus adult plants growing in the wild,the expression levels of AmRD22 were markedly high from the midautumn to the midwinter compared to those in other seasons.(3)Transgenic Arabidopsis with AmRD22 gene showed significantly enhanced salt tolerance and lowered Na+content.The transgenics also showed improved drought tolerance and reduced ABA sensitivity at early seed germination stage but had no obvious change in chilling and freezing tolerances.

关 键 词：沙冬青 RD22 基因表达 转基因 耐逆性 

分 类 号：Q785[生物学—分子生物学] Q786