同株紫斑牡丹中单瓣与重瓣花芽分化及内源激素含量比较  

Comparison of Flower Bud Differentiation and Endogenous Hormone Contents between Single and Double Petals of Paeonia rockii from Same Plant

作　　者：张瑞 唐红[1] 何丽霞 ZHANG Rui;TANG Hong;HE Lixia(Academy of Forestry Gansu Agricultural University,Lanzhou 730070,China;Gansu Engineering Research of Peony,Lanzhou 730046,China)

机构地区：[1]甘肃农业大学林学院,兰州730070 [2]甘肃省牡丹工程研究中心,兰州730046

出　　处：《西北植物学报》2022年第7期1152-1160,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：甘肃省牡丹工程研究中心项目(XZ20181120);甘肃省科技厅科技支撑计划(1604NKCA053)。

摘　　要：该研究以紫斑牡丹‘关公红’为试验材料,通过调查‘关公红’的开花情况,采用光学显微镜、扫描电镜技术考察花芽分化和花瓣发育过程的结构特征,并测定同株‘关公红’单、重瓣花朵的花芽在不同分化阶段内源激素含量,分析单、重瓣花朵的花芽分化及内源激素含量的变化特征,为紫斑牡丹不同花型的培育奠定理论基础。结果表明:(1)‘关公红’单瓣花朵比重瓣花朵花期长,单瓣花朵花瓣表皮细胞光滑平整无明显褶皱,而重瓣花朵花瓣的表皮有明显的褶皱;重瓣花的花芽分化时间明显早于单瓣,花瓣原基的体积也明显增大,这可能与重瓣花中雄蕊瓣化有关系。(2)低浓度GA_(3)有利于‘关公红’单瓣花朵和重瓣花朵的花芽分化;高浓度的ABA促进重瓣花原基的形成;ZR含量增加有助于紫斑牡丹的花芽分化。(3)较高ZR/IAA、ZR/GA_(3)、ABA/GA_(3)、ABA/IAA比值有利于‘关公红’重瓣花朵的花芽分化;ABA/IAA、ZR/IAA比值的增加有利于‘关公红’单瓣花朵花芽分化;(GA_(3)+IAA+ZR)/ABA与(IAA+ZR)/GA_(3)比值减小分别促进重瓣花朵花瓣原基和雄蕊原基的形成。In order to understand the characteristics of flower bud differentiation and endogenous hormone content of the single and double flowers of the same plant of Paeonia rockii‘Guangonghong’,and to lay a theoretical foundation for the cultivation of different flower types of Paeonia rockii,we used the Paeonia rockii‘Guangonghong’as the test material to investigate the flowering of‘Guangonghong’.Optical microscopy(OM)and scanning electron microscopy(SEM)were used to study the structural characteristics of flower bud differentiation and petal development.Contents of endogenous hormones in flower buds of single and double flowers of the strain‘Guangonghong’at different differentiation stages.The result shows:(1)the single-petal flower of‘Guangonghong’has a longer flowering period than the double petal flower.The epidermal cells of the single-petal flower petal are smooth and flat without obvious wrinkles,while the epidermis of the double-petal flower petal has obvious folds.The flower bud differentiation time of the double flower was significantly earlier than that of the single flower,and the volume of the petal primordia was also significantly increased,which may be related to the stamen petalization in the double flower.(2)Low concentration of GA_(3) is beneficial to the flower bud differentiation of single and double flowers of‘Guangonghong’;high concentration of ABA promotes the formation of double flower primordia;the increase of ZR content is conducive to the flower bud differentiation of Paeonia suffruticosa.(3)Higher ZR/IAA,ZR/GA_(3),ABA/GA_(3),and ABA/IAA are beneficial to the flower bud differentiation of double-petaled flowers of‘Guangonghong’;the increase of ABA/IAA and ZR/IAA ratios are beneficial to flower bud differentiation of single-petaled flowers of‘Guangonghong’;the decrease of(GA_(3)+IAA+ZR)/ABA and(IAA+ZR)/GA_(3) ratios promoted the formation of petal primordia and stamen primordia,respectively.

关 键 词：紫斑牡丹 花表型差异 重瓣花 花芽分化 内源激素 

分 类 号：Q944.58[生物学—植物学] Q945.6[农业科学—观赏园艺] S685.11[农业科学—园艺学]