高温胁迫对萝卜幼苗生长及相关基因表达的影响  

Effect of High Temperature Stress on Growth and Expression Levels of Related Genes in Radish Seedling

作　　者：周娜[1] 郑阳[1] 陆景伟[1] 胡燕[1] 陶伟林[1] 雷开荣[2] 潘晓雪[1] ZHOU Na;ZHENG Yang;LU Jingwei;HU Yan;TAO Weilin;LEI Kairong;PAN Xiaoxue(Vegetable and Flower Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;Chongqing Key Laboratory of Adversity Agriculture,Biotechnology Research Institute/Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆市农业科学院蔬菜花卉研究所,重庆401329 [2]重庆市农业科学院生物技术研究所/逆境农业研究重庆市市级重点实验室,重庆401329

出　　处：《西北植物学报》2022年第7期1161-1168,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家大宗蔬菜产业体系重庆综合试验站(CARS-25-G-40);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0143)。

摘　　要：该研究以耐热性较强的11162-3-1T-1和对热敏感的Wr129A2A1萝卜高代自交系为试材,对比分析40℃高温处理对萝卜幼苗的生长表型、光合特性、渗透调节、抗氧化能力以及热激胁迫相关基因表达情况的影响,以揭示萝卜苗期对高温胁迫的生理响应机制。结果表明:(1)在田间自然高温下,11162-3-1T-1长势正常,Wr129A2A1长势较弱、越夏死株率100%;人工40℃高温胁迫处理24 h后,11162-3-1T-1仍然保持绿色,在适温条件下恢复生长1周后全部成活,而Wr129A2A1脱水严重,植株萎蔫、干枯变黄、甚至整株枯死,适温下无法恢复。(2)两个萝卜品系苗期叶片光合系统Ⅱ的最大光化学量子产量(F_(v)/F_(m))和叶绿素含量在高温胁迫下均减少,但11162-3-1T-1的下降幅度明显较小。(3)在苗期高温胁迫下,两个品系叶片MDA和H_(2)O_(2)含量均升高,但11162-3-1T-1增幅明显较小;超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性以及可溶性糖含量均呈不同程度增加,但11162-3-1T-1增幅更大;期间两个材料叶片中脯氨酸含量均未见明显变化。(4)在苗期高温胁迫下,两个品系叶片中热激胁迫相关基因RsHSP101、RsAPX2、RsSUS1、RsHSP25.3、RsHsfA2和RsGolS1表达量均呈不同程度增加,但11162-3-1T-1增幅更大。研究发现,耐热性萝卜品系11162-3-1T-1在苗期高温胁迫下的生长表型、光合特性、渗透调节能力、抗氧化能力以及热激胁迫相关基因表达等方面均比热敏感材料表现更佳,可作为培育优质早熟耐热萝卜新品种优良种质资源,该研究同时为萝卜耐高温机制解析提供了理论依据。In order to reveal the physiological response to high temperature stress in radish,we used the seedlings of two different radish cultivars,11162-3-1 T-1(heat-tolerance)and Wr129 A2 A1(heat-sensitive)to compare the growth phenotype,photosynthetic characteristics,osmotic regulation,antioxidant capacity,and the expression levels of stress relative genes under 40℃treatments.The results showed that:(1)upon exposure to natural high temperature stress,the susceptible genotype,Wr129 A2 A1 turned weak and died;whereas the resistant genotype,11162-3-1 T-1 remained normal in growth;At 40℃,11162-3-1 T-1 remained green and was able to recover after two weeks in regular growth conditions at seedling stage,whereas Wr129 A2 A1 was completely wilted and died.(2)The F_(v)/F_(m) value and chlorophyll contents of leaves were decreased in both cultivars under high temperature stress,but 11162-3-1 T-1 was lose significantly lower than Wr129 A2 A1.(3)The H_(2)O_(2) and MDA contents were increased in both cultivars under high temperature stress,11162-3-1 T-1 was significantly lower than Wr129 A2 A1;at the same time,a relative significantly higher SOD and APX activities,soluble sugar content had been found in 11162-3-1 T-1.However,the proline content was no significant difference between Wr129 A2 A1 and 11162-3-1 T-1 at seedlings stages.(4)Compared with Wr129 A2 A1,the relative expression of RsHSP101,RsAPX2,RsSUS1,RsHSP25.3,RsHsfA2 and RsGolS1 were significant increase in 11162-3-1 T-1.The study found that the heat-tolerant 11162-3-1 T-1 performed better than the heat-sensitive material in terms of growth phenotype,photosynthetic characteristics,osmotic regulation,antioxidant capacity,and the expression of heat stress related genes under high temperature condition in radish seedlings,and provide germplasm resources for breeding high-quality heat-tolerant and early-maturing varieties and at the same time provide a theoretical basis for the analysis of heat-tolerant mechanism of radish.

关 键 词：萝卜 高温胁迫 苗期 耐热性 调控机制 

分 类 号：Q945.78[生物学—植物学] Q789