5种外源物质对干旱胁迫下笔筒树幼苗生长的缓解效应  

Alleviation Effect of Five Exogenous Substances on Sphaeropteris lepifera Seedlings under Drought Stress

作　　者：刘鑫 付丽娟 于静 郑钠元 刘保东[1] 丁国华[1] LIU Xin;FU Lijuan;YU Jing;ZHENG Nayuan;LIU Baodong;DING Guohua(School of Life Science and Technology,Harbin Normal University,Harbin 150025,China;Fengtai School,High School Affiliated to Renmin University of China,Beijing 100074,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025 [2]中国人民大学附属中学丰台学校,北京100074

出　　处：《西北植物学报》2022年第7期1169-1179,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31770350);国家重点实验室开放课题(K2018204)。

摘　　要：为探究外源物质对干旱胁迫下笔筒树生长的缓解效应和生理机制,对实验室培养的笔筒树幼苗进行了自然干旱胁迫,期间分别叶面喷施1.0 mmol/L水杨酸(SA)、150μmol/L褪黑素(MT)、100 mg/L多效唑(PP_(333))、2.5 mmol/L氯化钙(CaCl_(2))以及0.3 mg/L的2,4-表油菜素内酯(EBR)溶液,测定各处理幼苗的生长和光合作用、抗氧化酶、渗透调解物质等相关生理指标。结果表明:(1)与正常生长对照(CK)相比,干旱胁迫(DCK)显著抑制笔筒树幼苗地上部分的生长,但能够显著促进地下部分的生长;干旱胁迫显著抑制笔筒树叶片抗氧化酶活性,加剧对细胞膜的损害,进而引起幼苗叶片相对电导率和丙二醛含量显著上升,显著增加渗透调节物质的积累;干旱胁迫也使叶片叶绿素含量显著降低,不能正常进行光合作用,其净光合速率、气孔导度和蒸腾速率都显著降低。(2)与DCK处理相比,叶面喷施外源物质可以显著提高笔筒树幼苗叶片抗氧化酶活性,有效清除细胞内过多的活性氧,显著降低相对电导率和丙二醛含量,使细胞膜系统代谢正常,减轻干旱胁迫对笔筒树幼苗造成的伤害,并提高净光合速率、气孔导度、蒸腾速率和叶绿素含量,有效减轻干旱胁迫对光合作用的抑制。(3)隶属函数法综合评价表明,适宜浓度的水杨酸(SA)和褪黑素(MT)处理对笔筒树幼苗干旱胁迫的缓解效应最好。To explore the mitigation effect and physiological mechanism of exogenous substances on the growth of Sphaeropteris lepifera under drought stress,we cultured the S.lepifera seedlings in the laboratory were subjected to natural drought stress,and sprayed the leaves with 1.0 mmol/L SA solution,150μmol/L MT solution,100 mg/L PP_(333) solution,2.5 mmol/L CaCl_(2) solution and 0.3 mg/L EBR solution to determine the growth,photosynthesis,antioxidant enzymes,osmotic mediators and other related physiological indexes of seedlings.The results showed that:(1)compared with normal growth control(CK),drought stress significantly inhibited the growth of aboveground part and significantly promoted the growth of underground part of DCK;drought stress also significantly inhibited the activities of antioxidant enzymes in leaves,aggravated the damage to the cell membrane,and caused a significant increase in the electrolyte and MDA content in the leaves of the S.lepifera seedlings,and also significantly increased the accumulation of osmotic regulators;drought stress also significantly reduced the chlorophyll content in the leaves,which could not carry out normal photosynthesis,and its net photosynthetic rate,stomatal conductance and transpiration rate were significantly reduced.(2)Compared with DCK,foliar spraying of exogenous substances can significantly improve the antioxidant enzyme activities of seedling leaves,effectively remove excessive reactive oxygen species in cells,significantly reduce the relative conductivity and malondialdehyde content,make the metabolism of cell membrane system normal,reduce the damage caused by drought stress to S.lepifera seedlings,and improve the net photosynthetic rate,stomatal conductance,transpiration rate and chlorophyll content,effectively reduce the inhibition of drought stress on photosynthesis.(3)The comprehensive evaluation of membership function method showed that the treatment of appropriate concentration of salicylic acid(SA)and melatonin(MT)had the best mitigation effect on drought str

关 键 词：笔筒树 干旱胁迫 外源物质 缓解效应 

分 类 号：Q945.78[生物学—植物学]